| 引用本文:焦海燕，严志祎，马庆宇，周岩，李晓娟，潘秋霞，王亭晔，刘玥芸，陈家旭.逍遥散对肝郁脾虚证模型大鼠海马TPH2与IDO1的调节作用[J].世界中医药,2017,(03):. |
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| 逍遥散对肝郁脾虚证模型大鼠海马TPH2与IDO1的调节作用 |
| Influence of xiaoyaosan on the expression of TPH2 and IDO1 in hippocampus of rat with syndrome of liver depression and spleen deficiency |
| 投稿时间：2017-02-20 |
| DOI：10.3969/j.issn.1673-7202.2017.03.004 |
| 中文关键词: 肝郁脾虚证 逍遥散 TPH2 IDO1 |
| English Keywords:Syndrome of liver depression and spleen deficiency Xiaoyaosan TPH2 IDO1 |
| 基金项目:国家自然科学基金面上项目（编号：81473597）；国家自然科学基金重点项目（编号：81630104）；北京中医药大学自主课题(编号：2016-JYB-XS012) |
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| 中文摘要: |
| 目的：观察肝郁脾虚证模型大鼠海马色氨酸羟化酶2（TPH2）与吲哚胺-2,3-双加氧酶1（IDO1）表达的变化并探讨逍遥散的调节作用。方法：雄性SD大鼠随机分成4组，分别是正常组、模型组、逍遥散组、氟西汀组，每组6只。采用慢性束缚应激的方法制备大鼠肝郁脾虚证模型，造模持续21 d。通过荧光定量PCR与Western-blot方法检测各组大鼠海马TPH2与IDO1的表达情况。结果：模型组大鼠海马TPH2 mRNA的表达水平低于其余3组大鼠（P<0.05），逍遥散与氟西汀对TPH2 mRNA的表达有一定的上调作用；模型组大鼠海马IDO1 mRNA的表达显著增加（P<0.01），逍遥散与氟西汀对IDO1 mRNA表达的下调作用明显（P<0.01），且逍遥散对IDO1 mRNA的下调作用更明显。模型组、逍遥散组、氟西汀组大鼠海马TPH2的蛋白的表达低于正常组（P<0.01），逍遥散组TPH2蛋白表达高于模型组（P<0.05）；模型组大鼠海马IDO1的蛋白表达显著高于其余3组（P<0.01）。结论：肝郁脾虚证模型大鼠海马TPH2的表达减少，IDO1的表达增多，逍遥散可能通过调节海马TPH2与IDO1的表达水平进而影响5-HT的含量，起到治疗作用。 |
| English Summary: |
| To observe the expression of TPH2 and IDO1 in hippocampus of rat with syndrome of liver depression and spleen deficiency, and to explore the regulating effect of xiaoyaosan. Methods:Male SD rats were randomly divided into 4 groups：normal group, model group, xiayaosan group and fluoxetine group, each including 6 rats. The liver depression and spleen deficiency model was established by chronic immobilization stress for 21 days. The expression of TPH2 and IDO1 were detected by Fluorescent quantitative PCR and Western-blot method. Results:The mRNA expression of TPH2 in hippocampus of model group decreased significantly compared with the other groups (P<0.05); xiaoyaosan and fluoxetine could increase the TPH2 mRNA expression. The mRNA expression of IDO1 in hippocampus of model group increased significantly (P<0.01); xiaoyaosan and fluoxetine had the down-regulatory effect on IDO1 mRNA expression (P<0.01), which is more obvious in xiaoyaosan group. The protein expression of TPH2 in normal group was higher than the other groups (P<0.01), which of xiaoyaosan group was higher than model group (P<0.05); the protein expression of IDO1 in model group increased significantly compared with the other groups (P<0.01). Conclusion:The expression of TPH2 decreases and IDO1 increases in liver depression and spleen deficiency model rats, and xiaoyaosan has a therapeutic effec by regulating TPH2 and IDO1 in hippocampus to influence the content of 5-HT. |
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