| 引用本文:李生斌1，赵峥1，万娇1，赵惠1，刘爱玲1，白媛媛1，刘春军2.不同炮制时间下的西洋红参对免疫力低下小鼠免疫功能的影响[J].世界中医药,2017,(03):. |
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| 不同炮制时间下的西洋红参对免疫力低下小鼠免疫功能的影响 |
| Effects of Panax quinquefolium L.Water Extraction under Different Processed Time on Hypoimmunity in Mice |
| 投稿时间：2016-04-27 |
| DOI：10.3969/j.issn.1673-7202.2017.03.037 |
| 中文关键词: 炮制 西洋参 免疫力低下 |
| English Keywords:Panax quinquefolium L. Processed Hypoimmunity |
| 基金项目: |
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| 中文摘要: |
| 目的：探讨不同炮制时间下西洋红参水提物对对免疫力低下小鼠免疫功能的影响。方法：清洁级小鼠70只，随机平均分为空白组、阳性药组、模型组、2 h炮制组、4 h炮制组、6 h炮制组及生药组，每天分别给予等量生理盐水、左旋咪唑（30 mg/kg）、2 h炮制的西洋红参水提物（200 mg/kg）、4 h炮制的西洋红参水提物（200 mg/kg）、6 h炮制的西洋红参水提物（200 mg/kg）及生药水提物（200 mg/kg）。对除空白组外小鼠采用环磷酰胺（50 mg/kg）腹腔给药诱导小鼠免疫低下模型。分别检测脾脏及胸腺指数；应用碳廓清实验、ConA与LPS诱导小鼠脾淋巴细胞增殖反应；检测各组小鼠T淋巴细胞亚群值。结果：各种炮制时间的西洋红参及生药均可不同程度的提高免疫低下小鼠的脾脏及胸腺指数、廓清指数、吞噬指数、脾淋巴细胞增殖反应、CD4+及CD8+细胞数，且可升高CD4+/CD8+细胞比值。在各炮制组中，2 h炮制的西洋红参水提物免疫调节功能优于其余炮制组及生药组。结论：不同炮制时间的西洋红参水提物对免疫力低下小鼠的免疫调节作用均不相同，其中2 h炮制时间活性最佳，值得进行深入研究。 |
| English Summary: |
| To explore the effects of Panax quinquefolium L.under different processed time on hypoimmunity in mice.Methods:Seventy KM mice，randomly divided into a blank control group and positive drug group，model group，2 h processed group，4 h processed group，6 h processed group and crude drug group，were given same amount of normal saline，levomisole (30 mg/kg)，2 h extract (200 mg/kg)，4 h extract (200 mg/kg)，6 h extract (200 mg/kg) and crude drug extract (200 mg/kg) each day，respectively.Except the blank group，the other mice were treated by cyclophosphamide (50 mg/kg) through abdominal administration to induce low immune mice model.To detect the spleen and thymus index，indexes of the clearance rate of carbon，ConA and LPS were applicated to induce spleen lymphocyte proliferation response，testing each value of T lymphocyte subsets in mice.Results:All kinds of processing time of ginseng and crude drugs can influence the levels of spleen and thymus index，expurgation index，index，spleen lymphocyte proliferation reaction and the number of CD4+ and CD8+ cells，and can increase the ratio of CD4+ / CD8+ cells in the lower immune mice.In each processing group，2 h group was more active than the other groups.Conclusion:Different processing time of Panax quinquefolium L.water extract on hypoimmunity in mice varies in effect，in which 2 h processed time has the best activity and is worth of further research. |
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