To explore the therapeutic effect of Xiaoyao Pill on the sub-healthy people and to analyze its effect on immune function and endocrine system. Methods:Fifty-seven sub-healthy patients diagnosed with liver qi stagnation type who had treatment in the hospital from November 2014 to February 2016 were selected and included into the treatment group，treated with oral Chinese patent medicine Xiaoyao Pill. Fatigue assessment instrument(FAI) and self-rating anxiety scale(SAS) were applied for assessment and changes in serum level of immunoglobulin A (IGA)，T lymphocytes (CD3+，CD4+)，plasma norepinephrine (NE)，epinephrine (E) were detected before and after the treatment and physical examination of healthy adult volunteers were compared as the control group. Results:After treatment，fatigue scores in the treatment group decreased significantly (P<0.05). The level of serum IgA，CD3+，CD4+，NE level improved to some degree and CD3+，CD4+ increased more significantly (P<0.05). E significantly reduced (P<0.01). Conclusion:Xiaoyao Pill can effectively regulate the endocrinal level of sub-healthy people and improve the immune function of the body.