To observe the effects of different intervention orders of electroacupuncture and rehabilitation on the expression of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) in patients with traumatic brain injury (TBI). Methods:A total of 74 cases of TBI patients(admitted from June 2015 to October 2016 in our hospital) were selected and randomly divided into control group and experimental group， with 37 cases in each. The control group was treated with electroacupuncture and rehabilitation successively， and the experimental group was treated with rehabilitation and electroacupuncture successively. The curative effect and the level of TNF-α， IL-1β in the two groups were compared using the Fugl-Meyer scale (FMA). Besides， the upper and lower limb motor function of the two groups were evaluated. Results:After treatment， Barthel index， MMSE scores of two groups were significantly higher， and the NIHSS score was significantly lower (P<0.01). After treatment， NIHSS score of experimental group was significantly higher than that of the control group (P<0.05)， but there is no significant difference in other scores of the two groups (P>0.05). After treatment， upper and lower limb motor function (FMA score) of the two groups were significantly higher (P<0.01)， and that of the experimental group was higher than that of the control group (P<0.01). Moreover， levels of TNF-α， IL-1β in the two groups were significantly lower (P<0.01)， there is no significant difference between the two groups (P>0.05). Conclusion:Both treatment can improve the clinical symptoms of TBI patients， reduce the level of TNF-α， IL-1β， reduce the damage of brain tissue， and improve the function of limb motor. However， TBI patients treated with electroacupuncture-rehabilitation order can recover more quickly.