| 引用本文:崔云华1，沈文宾1，吴焕淦1，周次利1，雷菲1，黄立师1，赵琛2，李璟3.艾灸对亚急性衰老大鼠胸腺CD4+T细胞IL-2、IL-2Rα、NF-κB和I-κB蛋白表达的影响[J].世界中医药,2017,(05):. |
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| 艾灸对亚急性衰老大鼠胸腺CD4+T细胞IL-2、IL-2Rα、NF-κB和I-κB蛋白表达的影响 |
| Effects of Moxibustion on the Expression of IL-2, IL-2Rα, NF-κB and I-κB in Thymus CD4+T Cells of Subacute Aging Rats |
| 投稿时间：2017-04-19 |
| DOI：10.3969/j.issn.1673-7202.2017.05.036 |
| 中文关键词: 亚急性衰老 IL-2 IL-2Rα NF-κB I-κB 艾灸 |
| English Keywords:Subacute aging IL-2 IL-2Rα NF-κB I-κB Moxibustion |
| 基金项目:国家重点基础研究发展计划(“973”计划)资助项目(编号:2009CB522900;2015CB554501);国家自然科学基金项目(编号:81303029;81373752) |
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| 中文摘要: |
| 目的:通过观察IL-2及相关受体蛋白水平的变化情况,探讨艾灸对亚急性衰老大鼠胸腺CD4+T细胞的影响。方法:采用D-半乳糖致亚急性衰老大鼠模型。24只清洁级雄性SD大鼠随机分为:正常组,模型组和艾灸组。艾灸组温和灸双侧肾俞穴5 min/次,共计40 d。采用免疫磁珠法分离胸腺CD4+T细胞,并再用Western Blot方法检测各组大鼠胸腺CD4+T细胞IL-2、IL-2Rα、NF-κB和I-κB的蛋白表达量。结果:模型组大鼠胸腺CD4+T细胞中IL-2、IL-2Rα、NF-κB、I-κB蛋白的表达量较正常组降低,差异有统计学意义(P<0.05);艾灸组大鼠胸腺CD4+T细胞中IL-2、IL-2Rα、NF-κB、I-κB蛋白表达量升高,与模型组比较差异有统计学意义(P<0.05)。结论:艾灸肾俞穴可上调胸腺CD4+T细胞IL-2、IL-2Rα、NF-κB、I-κB的表达。 |
| English Summary: |
| To observe the effect of moxibustion on CD4+T cells of thymus in subacute aging rats by recording the changes of IL-2 and related receptor protein levels. Methods:Use D-galactose induced subacute aging rat model. Twenty-four male SD rats were randomly divided into normal group, model group and moxibustion group. In moxibustion group, use mild moxibustion on Shenshu Point(BL23) bilateral for 40 days, 5 minutes each day each time. To separate CD4+T cells by MACS method, and access the expression of IL-2, IL-2Rα, NF-κB and I-κB in CD4+T cell by Western Blot. Results:Western Blot assay shows that comparing to the control group, the expression of IL-2, IL-2Rα, NF-κB and I-κB in CD4+T cell of model group significantly decreased (P<0.05). However, in the moxibustion group, those increased with significant difference (P<0.05). Conclusion:In subacute aging rats, moxibustion on BL23 can improve the expression of IL-2, IL-2Rα, NF-κB and I-κB in thymic CD4+T cell. |
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