To explore the effects of Astragalus glycoproteins on collagen-induced arthritis mice's specific transcription factors, T-bet and GATA-3, expression, and to clarify the immune mechanism of Astragalus glycoprotein on the treatment in collagen-induced arthritis mice. Methods:CIA model was induced by bovine Ⅱcollagen in mice. Mice were randomly divided into a CIA model group, a hydrocortisone positive control group, a Astragalus glycoprotein low dose group, a middle dose group and a high dose group. HE was used to observe the CIA mice spleen tissues damage. IFN-γ and IL-4 level were measured with flow cytometry in CIA mice. Western blot was used to analyze T-bet and GATA-3 protein expression. Results:Compared with the model group, Astragalus glycoprotein improved spleen tissue damage in CIA mice, and inhibited the infiltration of inflammatory cells. Astragalus glycoprotein reduced IFN-γand IL-4 level (P<0.05) and T-bet and GATA-3 protein expression (P<0.05). Conclusion:The mechanisms of action of Astragalus glycoprotein on CIA mice may be related to the decrease of T-bet and GATA-3 expression and function balance of the Th1 and Th2 cells.