To observe the effect of Wen Xin Dan on myocardial enzyme indicators, including CK-MB, cTnI and tumor necrosis factor alpha (TNF alpha) levels, and interleukin 6 (IL-6) of MIRI rats. Besides, to explore its effect on inflammatory factor of MIRI rats. Methods:A total of 70 SD rats were randomly divided into 7 groups with 10 in each. Groups include control group, model group, pretreatment group, Wen Xin Dan1:1:1 group, Wen Xin Dan1:2:1 group, Wen Xin Dan1:1:2 group, Wen Xin Dan2:1:1 group. After 14 days lavage, ligate rat's left anterior descending coronary artery (LAD). Again, lavage the rats to make myocardial ischemia models. Use automatic biochemical instrument to measure CK-MB content and cTnI ELISA method to measure TNF alpha, IL-6 levels. Results:Serum CK-MB, cTnI levels increased significantly in model group with significant difference comparing to those of the control group (P<0.01). Compared to the model group, myocardial enzyme index in each Wen Xin Dan group has changed, and the difference is significant (P<0.01), among which, CK-MB in the 1:2:1 group reduced most significantly. Serum TNF alpha, IL-6 levels increased significantly in the model group with significant difference especially compared to those of the control group (P<0.01). Compared with model group, TNF alpha and IL-6 content has changed significantly in all Wen Xin Dan group (P<0.01), among which the 1:2:1 group has the largest decrease. Conclusion:Wen Xin Dan can reduce serum CK-MB, cTnI and the content of TNF alpha, IL-6, therefore, it has anti-inflammatory effects.