世界中医药

目的：观察姜黄素对颈动脉斑块稳定性及内祖皮细胞（EPCs）的影响。方法：将30只新西兰兔随机分为空白对照组、模型组及姜黄素组，各10只，其中空白对照组予普通饲料喂养，模型组予喂养高脂饲料，姜黄素组予喂养高脂饲料+100 mg/d姜黄素。连续喂养12周后HE染色法观察3组西兰兔主动脉内膜斑块厚度、外周血总胆固醇（TC）、三酰甘油（TG）、低密度脂蛋白（LDL-C）、高密度脂蛋白（HDL-C）水平以及利用MTT法测定人纤维EPCs细胞迁徙及增殖活性。结果：1）血脂水平：与空白组比较，模型组及姜黄素组TC、TG及LDL-C均升高（P<0.05），HDL-C降低（P<0.05），其中姜黄素干预后上述指标有明显改善（P<0.05）。2）形态学变化：空白对照组颈动脉壁厚薄均匀，结构均匀，模型组及姜黄素均出现动脉管腔狭窄，动脉内膜增厚，其中姜黄素组较模型组改善。3）EPCs增殖情况：与空白组比较，模型组及姜黄素OD值均降低（P<0.05），与模型组比较，姜黄素OD有所上调（P<0.05）。4）EPCs迁徙能力：空白对照组各时间点划痕愈合率均显著高于其余2组（P<0.05），其中姜黄素组各时间点划痕愈合率均高于模型组（P<0.05）。结论：姜黄素由明显抗动脉粥样硬化作用，其作用机制可能与促进EPCs增殖及迁徙有关。

English Summary:

To observe the influence of curcumin on stability of rabbit carotid plaques and endothelia progenitor cells （EPCs）.Methods: Thirty New Zealand rabbits were randomly divided into a blank control group, a model group and a curcumin group, 10 cases in each. The blank control group had ordinary diet, the model group high fat diet, the curcumin group high fat diet plus 100 mg/d of curcumin. After continuous feeding for 12 weeks, thickness of aortic intima plaque, peripheral blood total cholesterol （TC）, triglyceride （TG）, low density lipoprotein （LDL-C）, high density lipoprotein （HDL-C） levels of all rabbits were observed by HE staining and measured migration and proliferation activity of EPCs cell by MTT method. Results: 1） Lipid levels: compared with the blank group, TC, TG, and LDL-C were higher in the model group and the curcumin group （P<0.05）, HDL-C lower （P<0.05）, and after the intervention of curcumin, the above indicators improved significantly （P<0.05）. 2） Morphological changes: the thickness of carotid artery wall in the blank control group were uniform, and with uniform structure, and the model group and the curcumin group were with artery luminal stenosis, thickening of the arterial intima, and the curcumin group improved compared with the model group. 3） The EPCs proliferation: compared with the blank group, OD values of the model group and the curcumin group were lower （P<0.05）; compared with the model group, OD of the curcumin group increased （P<0.05）.4） Capacity of EPCs migration: scratches healing rate of the blank control group at each time point were significantly higher than that of the other two groups （P<0.05）; the scratch healing rate of the curcumin group was higher than that of the model group at each time point （P<0.05）. Conclusion: Curcumin had obvious resistance effect on atherosclerosis, and the mechanism may be associated with the promoting of proliferation and migration on the EPCs.

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