| 引用本文:吴国学1,2，张振凌2.不同种类酒炮炙对牛膝饮片指纹图谱的影响[J].世界中医药,2017,(06):. |
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| 不同种类酒炮炙对牛膝饮片指纹图谱的影响 |
| Effect of Different Types of Ethanol Processing Method on Fingerprint of Medicinal Cyathula Root |
| 投稿时间：2017-02-08 |
| DOI：10.3969/j.issn.1673-7202.2017.06.058 |
| 中文关键词: 牛膝 指纹图谱 酒炙 HPLC 蜕皮甾酮 不同浓度乙醇 |
| English Keywords:Medicinal Cyathula Root Fingerprints Ethanol processing HPLC Ecdysterone Different concentrations of ethanol |
| 基金项目:国家“十一五”科技支撑计划项目（编号：2006BAI09B06-07） |
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| 摘要点击次数: 986 |
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| 中文摘要: |
| 目的：比较牛膝生品、蒸馏水炙品及不同乙醇浓度酒炙品指纹图谱差异，研究不同乙醇浓度酒炮炙对牛膝饮片的影响。方法：采用HPLC，以岛津VP-ODS（4.6 mm×250 mm，5 μm）为色谱柱，乙腈-水为流动相梯度洗脱，流速0.8 mL/min，柱温25 ℃，检测波长243 nm，进样量15 μL，采集90 min。结果：牛膝各酒炙品大部分峰面积较生品的要稍高，蒸馏水炙品的较生品稍低，但不是很明显。结论：酒炙有利于本实验建立指纹图谱中的牛膝大部分有效成分的溶出，高浓度乙醇炙品在有效成分溶出方面优于低浓度乙醇炙品。 |
| English Summary: |
| To compare the fingerprints among Medicinal Cyathula Root raw products, distilled by solid water and different concentrations of ethanol, so as to stud the effects of Medicinal Cyathula Root products processed by different concentration of ethanol. Methods:By using HPLC method, taking the SHIMADZU VP-ODS （4.6×250 mm, μm） as the chromatographic column, acetonitrile-water as mobile phase gradient elution, flow rate 0.8 mL/min, column temperature 25 ℃,detection wavelength 243 nm, sample volume 15 μL, acquisition for 90 min. Results:Most of the peak area of Medicinal Cyathula Root that processed by ethanol were slightly higher than raw products; raw products were lower than the distilled water products, but not very obviously. Conclusion:In this experiment, Medicinal Cyathula Root processed by ethanol is conducive to the dissolution of most active ingredient, high concentration ethanol processed product is better than the low concentration ethanol in the terms of the active ingredients dissolution. |
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