To explore the mechanism of hepatoprotective effect of Jianpihuoxue Decoction on 24 hours acute alcohol intoxication rats and to discuss its mechanism of anti-oxidative stress. Methods:Twenty-eight Sprague Dawley rats were allocated to the normal group (8 rats), acute alcoholism model group (10 rats) and the jianpihuoxue decoction group (10 rats). Each group was given the jianpihuoxue decoction or water 1.0 mL/100 gwt twice a day for three days. The water and food were forbidden for 16 hours after the last administration three days later., then rats were given jianpihuoxue decoction or water 1.0 mL/100 gwt by gavage again.One hour later, acute alcoholism model group and the jianpihuoxue decoction group were given the same amount of 56% alcoholl 1.5 mL/100 gwt by gavage to reproduce the model of acute alcoholism. The serum and hepatic tissues were taken after 24 h to detected the related indexes. Results:Compared with the model group, Jianpihuoxue decoction reduced activities of serum ALT(P<0.01), alleviated microvesicular steatosis obviously and kupffer cells in the hepatocytes reduced, observed by H&E staining. The content of GSH of liver tissue increased significantly (P<0.01) while the expression of TNOS and iNOS lowered (P<0.05). Conclusion:Jianpihuoxue decoction protects the 24 hours acute alcoholism rats' livers and resists the oxidative stress damage by increasing the level of hepatic GSH, decreasing the level of hepatic iNOS.