To observe acute toxicity of Mangiferin for intragastric administration (I.g) at multiple dose in mice and dogs.Methods:Forty KM mice and six Beagle dogs were randomly divided into treatment group and control group.Intragastric administered maxi malad administration dose (MAD) based on approximate lethal dose (ALD) and animals in both groups were observed for 14 days.Results:All mice were alive and no abnormality was found in appearance,behavior and diet.In addition,no pathological changes were found after dissection.Grey feces were found once in dogs and the experiment group showed increase of TBIL,with an occurrence rate of 25%,but it restored to normal level after administration.No abnormality was found in blood and biochemical indicators.No physiological abnormality was found after dissection.Conclusion:No toxicity symptoms was found after oral administered 54 g/kg of mangiferin.One of four dog's TBIL increased significantly after given mangiferin MTD>9.0 g/kg,but it can restore to normal level.