| 引用本文:郝二伟1，2，王勤1，谭珍瑗3，王峥屹4，邓家刚1，2.芒果叶提取物芒果苷安全性评价Ⅳ：遗传、生殖毒性试验[J].世界中医药,2017,(07):. |
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| 芒果叶提取物芒果苷安全性评价Ⅳ：遗传、生殖毒性试验 |
| Safety Evaluation of Mangiferin Extracted from Mango Leaf—Genetic and Reproductive Toxicity Test |
| 投稿时间：2017-05-12 |
| DOI：10.3969/j.issn.1673-7202.2017.07.050 |
| 中文关键词: 芒果苷 遗传毒性 生殖毒性 致畸 |
| English Keywords:Mangiferin Genotoxicity Reproductive toxicity Teratogenic |
| 基金项目:广西重点实验室建设项目（15-140-31）；广西农作物废弃物功能成分研究协同创新中心建设（CICAR2015-Z1） |
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| 中文摘要: |
| 目的：检测芒果苷是否影响遗传物质，并通过口服灌胃给予妊娠期6~15 d致畸敏感期孕期大鼠芒果苷，考察其对妊娠动物、胚胎及胎仔发育的影响，观察芒果苷是否具有遗传和生殖毒性。方法：采用微生物回复突变试验、啮齿动物骨髓微核试验、哺乳动物培养细胞染色体畸变试验检测芒果苷的遗传毒性。从妊娠第7天开始，连续10 d，口服灌胃受试药品0.6 mg／(kg·bw)、1.2 mg／(kg·bw)、2.4 mg／(kg·bw)，观察其对各组孕鼠妊娠期毒性反应及孕鼠胚胎期各胎鼠生长发育指标致畸变的影响。结果：微生物回复突变试验中芒果苷各剂量组回变菌落数均在正常范围内未超过自发回变菌落数的2倍，骨髓微核试验中芒果苷各剂量组多染红细胞和正染红细胞比例未低于溶媒对照组的20%，染色体畸变试验中CHL细胞染色体畸变率均小于5%。芒果苷低、中、高3种不同剂量对各孕鼠及孕鼠胚胎期各胎鼠的窝胎数，胚胎形体外观、全身骨骼、各内脏重要脏器等的生长发育指标致畸变检测项目显示为阴性。结论：芒果苷无致突变性，对孕鼠胚胎期胎鼠生长发育指标未见有生殖毒性反应的致畸变影响。 |
| English Summary: |
| To evaluate the genetic and reproductive toxicity of mangiferin and observe its effects on genetic material,pregnant animals,embryos and fetuses.Methods:Genotoxicity test: The genetic toxicity of mangiferin was detected by microbial reversion mutation test,rodent bone marrow micronucleus test,mammalian cultured cell chromosome aberration test.Reproductive toxicity test: 0.6 mg/(kg·bw),1.2 mg/(kg·bw),2.4 mg/(kg·bw) of mangiferin were administered orally for 10 days from the first 7 days of gestation,in order to observe its toxicity on pregnant women and the the changes of growth and development indexes of fetal rats in embryos.Results:Genotoxicity test: The number of reversed colonies in each dosage group of mangiferin in the microbial reversion mutation test did not exceed 2 times of the number of spontaneous rejuvenation colonies in the normal range.In the bone marrow micronucleus test,the proportion of red blood cells was not lower than 20% of the vehicle control group,and chromosome aberration test CHL cell chromosome aberration rate was less than 5%.Reproductive toxicity test: in mangiferin low,medium and high dosage groups,the appearance of embryos,body bones,visceral organs and other important growth and development indicators were negative.The results showed that mangiferin was not mutagenic,and there was no reproductive toxicity on the growth and development of embryonic fetus in pregnant rats. |
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