To evaluate the genetic and reproductive toxicity of mangiferin and observe its effects on genetic material,pregnant animals,embryos and fetuses.Methods:Genotoxicity test: The genetic toxicity of mangiferin was detected by microbial reversion mutation test,rodent bone marrow micronucleus test,mammalian cultured cell chromosome aberration test.Reproductive toxicity test: 0.6 mg/(kg·bw),1.2 mg/(kg·bw),2.4 mg/(kg·bw) of mangiferin were administered orally for 10 days from the first 7 days of gestation,in order to observe its toxicity on pregnant women and the the changes of growth and development indexes of fetal rats in embryos.Results:Genotoxicity test: The number of reversed colonies in each dosage group of mangiferin in the microbial reversion mutation test did not exceed 2 times of the number of spontaneous rejuvenation colonies in the normal range.In the bone marrow micronucleus test,the proportion of red blood cells was not lower than 20% of the vehicle control group,and chromosome aberration test CHL cell chromosome aberration rate was less than 5%.Reproductive toxicity test: in mangiferin low,medium and high dosage groups,the appearance of embryos,body bones,visceral organs and other important growth and development indicators were negative.The results showed that mangiferin was not mutagenic,and there was no reproductive toxicity on the growth and development of embryonic fetus in pregnant rats.