世界中医药

作者	单位
张润田1，陈曦2，李玲玲1，杭海燕1，段行武1	1 北京中医药大学东直门医院，北京，100107； 2 北京中医药大学东直门医院东区，北京，101100

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中文摘要:

目的：探讨寻常型银屑病血热、血瘀、血燥证形成与皮肤组织中JAK1/STAT3信号通路的关系。方法：选取2013年10月至2014年10月东直门医院皮肤科门诊和病房收治的寻常型银屑病患者18例（严格中医辨证分为血热证、血瘀证、血燥证三型），正常人受试者6例，记录患者一般情况，用RT-PCR法检测各组受试者皮肤组织中IL-22R1、JAK1、STAT3、C-myc、VEGF的mRNA表达差异。结果：检测24例受试者皮肤组织，IL-22R1、JAK1、STAT3、C-myc的mRNA表达相互间均呈正相关，差异有统计学意义（P<0.05或P<0.01）；血热证患者皮损中，IL-22R1、JAK1、STAT3、C-myc的mRNA表达均明显高于正常人，差异有统计学意义（P<0.01），血燥证IL-22R1、JAK1、STAT3的mRNA表达高于正常人，差异有统计学意义（P<0.05）。结论：JAK1/STAT3信号通路的异常活跃，可能是银屑病血热证临床表现形成的重要因素；且JAK/STAT信号通路活化差异可能与血热证向血瘀、血燥证转变的过程相关。

English Summary:

To observe the differences between blood heat syndrome, blood dryness syndrome and blood stasis syndrome of psoriasis in JAK/STAT signaling pathways. Methods:A total of 18 psoriasis cases in our hospital were divided into 3 groups based on TCM syndrome differentiation. The three groups were blood heat syndrome group, blood dryness syndrome group and blood stasis syndrome group. And 6 healthy participants also participated in the trail. Their general information was recorded. RT-PCR was employed to test the difference of IL-22R1, JAK1, STAT3 and c-myc in participants' skin tissue samples. Results:The gene transcription correlation test among 24 psoriasis patients show that IL-22R1, JAK1, STAT3 and c-myc were all positively correlated to each other (P<0.05 or P<0.01）. The gene transcriptions of IL-22R1, JAK1, STAT3 and c-myc in blood heat group were significantly higher than healthy case group (P<0.01）. In blood dryness group, gene transcription of IL-22R1, JAK1 and STAT3 were higher than healthy group (P<0.05）. Conclusion:The active status of JAK/STAT signaling pathway could be an important factor in the formation of psoriasis blood heat syndrome. While the JAK/STAT signaling pathway may result in blood heat syndrome turning into blood dryness syndrome and blood stasis syndrome.

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