世界中医药

目的：研究分析犀角地黄汤对脓毒症大鼠肝保护作用的影响效果。方法：选择2016年9月在我院实验室中饲养的SPF级SD大鼠90只进行研究，根据简单数字随机表法将其分成观察组（制成脓毒症大鼠的模型后利用犀角地黄汤处理）、模型组（制成脓毒症大鼠的模型）、假手术组，每组30只。分别在手术后的8 h及16 h比较各组肝功能及炎性反应指标水平，肝组织匀浆的一氧化氮（NO）、丙二醛（MDA）、诱导型一氧化氮合酶（iNOS）及超氧化物歧化酶（SOD）水平，分析脓毒症大鼠谷丙转氨酶（ALT）及谷草转氨酶（AST）水平与NO、MDA、iNOS及SOD的相关性。结果：观察组及模型组术后8 h及16 h的ALT、AST、肿瘤坏死因子-α（TNF-α）及白细胞介素-6（IL-6）水平均分别明显高于假手术组，差异有统计学意义（P<0.05）。观察组术后8 h及16 h的ALT、AST、TNF-α及IL-6水平均分别明显高于模型组，差异有统计学意义（P<0.05）。观察组及模型组术后8 h及16 h的NO及iNOS水平均明显高于假手术组，SOD水平明显低于假手术组，术后16 h的MDA水平均分别明显高于假手术组，差异有统计学意义（P<0.05）。观察组术后8 h及16 h的NO及iNOS水平均明显低于模型组，术后16 h的MDA水平明显低于模型组，SOD水平明显高于模型组，差异有统计学意义（P<0.05）。依据Spearman法分析相关性可知，脓毒症大鼠ALT及AST水平与NO、MDA及iNOS均呈正相关，而与SOD呈负相关。结论：犀角地黄汤对脓毒症大鼠具有较好的肝保护作用，对于大鼠的肝功能及炎性反应症状存在较好的改善效果。

English Summary:

To study the influence of Xijiao Dihuang decoction on liver-protection in rats with sepsis. Methods:A total of 90 SPF SD rats were selected in the laboratory of our hospital in September 2016, they were randomly divided into 3 groups, the observation group (sepsis model rats with Xijiao Dihuang decoction treatment), model group (sepsis model rats), as well as sham operation group. There were 30 rats in each group. Liver function, inflammatory marker levels, nitric oxide (NO), malondialdehyde (MDA), inducible nitric oxide synthase (iNOS) and superoxide dismutase (SOD) in liver homogenate were compared among three groups 8 h and 16 h after surgery respectively. Besides, correlation between alanine aminotransferase(ALT) level as well as aspartate aminotransferase (AST) level and NO, MDA, iNOS, SOD was analized. Results:ALT, AST, tumor necrosis factor alpha (TNF-α) and interleukin-6 (IL-6) levels in the observation group and model group were significantly higher than those in sham operation group both after surgery 8 h and 16 h, the difference was statistically significant (P<0.05). The results in the observation group were significantly higher than those in model group, the difference was statistically significant (P<0.05) The iNOS and NO levels in the observation group and the model group were significantly higher than those in the sham operation group after 8 h and 16 h, while the SOD level was significantly lower than that in the sham operation group 16 h later the operation, the MDA level was higher than that in sham operation group, there was significantly difference. (P<0.05). The iNOS and NO levels in the observation group were significantly lower than those in the model group, MDA level after 16 h was significantly higher than that in model group, the level of SOD was significantly higher than that in model group, and the difference was statistically significant (P<0.05). According to the correlation analyzed by Spearman method, the levels of ALT and AST in septic rats were positively correlated with iNOS, MDA and NO, but negatively correlated with SOD. Conclusion:Xijiao Dihuang decoction is effective on liver in sepsis rats, and the symptoms of liver function and inflammation in rats really improves.

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