To study the influence of Xijiao Dihuang decoction on liver-protection in rats with sepsis. Methods:A total of 90 SPF SD rats were selected in the laboratory of our hospital in September 2016, they were randomly divided into 3 groups, the observation group (sepsis model rats with Xijiao Dihuang decoction treatment), model group (sepsis model rats), as well as sham operation group. There were 30 rats in each group. Liver function, inflammatory marker levels, nitric oxide (NO), malondialdehyde (MDA), inducible nitric oxide synthase (iNOS) and superoxide dismutase (SOD) in liver homogenate were compared among three groups 8 h and 16 h after surgery respectively. Besides, correlation between alanine aminotransferase(ALT) level as well as aspartate aminotransferase (AST) level and NO, MDA, iNOS, SOD was analized. Results:ALT, AST, tumor necrosis factor alpha (TNF-α) and interleukin-6 (IL-6) levels in the observation group and model group were significantly higher than those in sham operation group both after surgery 8 h and 16 h, the difference was statistically significant (P<0.05). The results in the observation group were significantly higher than those in model group, the difference was statistically significant (P<0.05) The iNOS and NO levels in the observation group and the model group were significantly higher than those in the sham operation group after 8 h and 16 h, while the SOD level was significantly lower than that in the sham operation group 16 h later the operation, the MDA level was higher than that in sham operation group, there was significantly difference. (P<0.05). The iNOS and NO levels in the observation group were significantly lower than those in the model group, MDA level after 16 h was significantly higher than that in model group, the level of SOD was significantly higher than that in model group, and the difference was statistically significant (P<0.05). According to the correlation analyzed by Spearman method, the levels of ALT and AST in septic rats were positively correlated with iNOS, MDA and NO, but negatively correlated with SOD. Conclusion:Xijiao Dihuang decoction is effective on liver in sepsis rats, and the symptoms of liver function and inflammation in rats really improves.