| 引用本文:王嘉麟，邢佳，贺立娟，朱晓晨，王椿野，王蕊，郑建颖，张迎，李秋祥，崔洪志，秦裕驹，符海燕，高维，郭晓，袁清洁，高东阳，郭凯航，王建伟，王玉来，郭蓉娟.益气活血药物对脑梗死恢复期大鼠c-fos基因表达的影响[J].世界中医药,2017,(09):. |
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| 益气活血药物对脑梗死恢复期大鼠c-fos基因表达的影响 |
| Effect of Supplementing Qi and Activating Blood Circulation Drugs on C-Fos Mrna of Rats with Cerebral Infarction |
| 投稿时间：2016-09-13 |
| DOI：10.3969/j.issn.1673-7202.2017.09.043 |
| 中文关键词: 脑梗死 c-fos 益气活血 |
| English Keywords:Cerebral infarction C-fos Supplementing qi and activating blood circulation |
| 基金项目:国家重点基础研究发展计划（973计划）(2015CB554401）；高等学校博士学科点专项科研基金资助课题(20120013120014）；北京中医药大学东方医院人才培养计划项目（东方新星）(DFRC2014C01） |
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| 中文摘要: |
| 目的：基于c-fos mRNA观察益气活血药物对脑梗死恢复期大鼠的影响。方法：以Longa的方法略作改良制作大鼠左侧大脑中动脉阻塞模型，按照随机数字表的方法随机分为正常组、模型组、假手术组、益气组、活血组、益气活血组、ERK抑制剂7组，采用RT-PCR技术，在治疗28 d观察益气活血药物对脑梗死恢复期大鼠梗死皮层c-fos mRNA表达的影响。结果：在治疗28 d后，模型组较正常组c-fos mRNA表达明显下降（P<0.05），益气活血组与ERK抑制组较模型组有一定程度提高（P<0.05），活血组与模型组比较，无明显统计学意义（P>0.05），益气组的c-fos mRNA表达低于其他各组（P<0.05）。结论：在脑梗死恢复期，益气活血药物在一定程度上可改善脑缺血，优于单纯益气与单纯活血，其疗效机制可能与提高c-fos mRNA的表达有关。 |
| English Summary: |
| To observe the expression of c-fos mRNA of rats with cerebral infarction in recovery period under supplementing qi and activating blood circulation drugs. Methods:Longa method was slightly modified in making the rat model of left middle cerebral artery occlusion. According to the random number table method，the rats were randomly divided into 7 groups，including a normal group, a model group, a sham operation group, a supplementing qi group, an activating blood circulation group, a supplementing qi and activating blood circulation group, and an ERK inhibitor group. Using RT-PCR technique, we observed the effect of supplementing qi and activating blood circulation drugs on c-fos mRNA of rats with cerebral infarction after 28 days' treatment in myocardial infarction cortex. Results:After 28 days' treatment，compared with normal group, c-fos mRNA expression in the model group was significantly decreased (P<0.05)；c-fos mRNA expression in the supplementing qi and activating blood circulation group and ERK inhibition group increased to a certain extent than that in the model group (P<0.05)；the activating blood circulation group had no significant statistical difference with the model group(P>0.05)；c-fos mRNA expression in supplementing Qi group was lower than the other groups (P<0.05). Conclusion:In the recovery period of cerebral infarction, supplementing qi and activating blood circulation drugs can improve cerebral ischemia in a certain degree, which is better than simply supplementing qi and activating blood circulation, and its therapeutic mechanism may be related to improving the expression of c-fos mRNA. |
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