世界中医药
文章摘要
引用本文:吴琼1,张淑静1,张宇晨1,邢恩龙1,杨傲然2,胡京红1,葛东宇1,黄翔1,田昕1.参知健脑方对缺氧致认知功能障碍大鼠脑线粒体损伤的保护机制研究[J].世界中医药,2017,(10):.  
参知健脑方对缺氧致认知功能障碍大鼠脑线粒体损伤的保护机制研究
Study on the Protective Mechanism of Shenzhi Jiannao Fang on Mitochondrial Injury of Rats with Cognitive Impairment Induced by Hypoxia
投稿时间:2016-12-25  
DOI:10.3969/j.issn.1673-7202.2017.10.033
中文关键词:  认知功能障碍  缺氧  线粒体  参知健脑方
English Keywords:Cognitive impairment  Hypoxia  Mitochondria  Shenzhi Jiannao Fang
基金项目:国家自然科学基金项目(81202684)
作者单位
吴琼1,张淑静1,张宇晨1,邢恩龙1,杨傲然2,胡京红1,葛东宇1,黄翔1,田昕1 1 北京中医药大学北京100029 2 首都医科大学附属北京康复医院北京100144 
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中文摘要:
      目的:从线粒体角度观察参知健脑方对缺氧致认知功能障碍大鼠线粒体损伤的保护机制的研究。方法:将老年大鼠随机分为3组,建立缺氧致线粒体损伤的认知功能障碍模型组以及参知健脑方(SZJ)干预组,并用Morris水迷宫对大鼠的学习记忆能力进行检测。采用酶联免疫吸附法(ELISA)检测线粒体细胞色素氧化酶(COX)的活性、8羟基脱氧鸟苷(8-OHdG)、β-淀粉样前体蛋白(βAPP)及β-淀粉样蛋白(Aβ1-42)的含量,观察4个指标与认知功能障碍之间的关联性,进而观察缺氧对模型组脑线粒体的影响及SZJ干预组对脑线粒体的保护作用。结果:持续低压缺氧造模脑组织中APP、Aβ1-42含量模型组与正常组、SZJ干预组间均有统计学意义(P<0.01),8-OHdG含量模型组与正常组、SZJ干预组间比较有统计学意义(P<0.01),SZJ干预组与正常组间比较有统计学意义(P<0.05),COX含量正常组与模型组间比较有统计学意义(P<0.01)、模型组与SZJ干预组比较有统计学意义(P<0.05)。结论:参知健脑方可以抑制APP、Aβ1-42的积累、DNA氧化损伤、增强脑线粒体COX的活性,从而保护线粒体发挥神经保护作用,进一步改善缺氧对认知功能障碍学习记忆能力的影响为参知健脑方的临床应用提供实验支持。
English Summary:
      To observe the protective mechanism of Shenzhi Jiannao (SZJN) on mitochondrial injury rats with cognitive impairment induced by hypoxia.Methods:The aged rats were randomly divided into three groups to establish cognitive impairment model groups of mitochondrial injury induced by hypoxia, and SZJN intervention group, and the learning and memory abilities of rats were detected by Morris water maze.ELISA was used to measure the activity of mitochondrial cytochrome oxidase (COX), and contents of 8-OHdG, β-APP and Aβ1-42.The correlation between the 4 indexes and cognitive dysfunction was observed to further observe the effect of hypoxia on the mitochondria of the model group and the protective effect of SZJN intervention group on cerebral mitochondria.Results:In brain tissue of models induced by continued hypobaric hypoxia, the contents of APP and Aβ1-42 in model group had significant difference when compared with normal group and SZJN intervention group.The content of 8-OHdG in model group was significantly different when compared with normal group and SZJN intervention group (P<0.01), and that of SZJN intervention group was significantly different when compared with normal group (P<0.05).There was a significantly difference in the content of COX when compared between normal group and model group (P<0.01), and was also significantly different between model group and SZJN intervention group (P<0.05).Conclusion:Shenzhi Jiannao Fang could inhibit the accumulation of APP, Aβ1-42 and DNA oxidative damage, strengthen the activity of mitochondrial COX, so that can protect the neuroprotective effect of mitochondria to further improve the effect of hypoxia on learning and memory of cognitive impairment, providing experimental support for clinical application of SZJN.
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