To investigate the influence of miRNA-146a gene disturbances and cinnamic aldehyde (CA) on TLR4，NO，and MMP-13，which was released by synovioblast，and to explore their roles in the molecular mechanism of synovial inflammation in osteoarthritis (OA).Methods:Plasmids of miR-146a gene mimics and inhibitors were loaded into LPS-induced FLS using lipofection transfection.Then the interferential FLS were treated with CA，and the release amount of TRL4，NO and MMP-13 were detected.Results:After the miRNA-146a related plasmid transfection，the variation tendency of the concentrations of TLR4，NO and MMP-13 were almost the same.Compared with control group，the inhibitor group was elevated，and mimics group was declined (P<0.05).Compared with the control group，when the interferential FLS was treated with CA alone，TLR4，its release of TLR4，NO and MMP-13 were significantly decreased (P<0.05)，whereas the mimics and CA coordinated intervention，the release was significantly decreased (P<0.05).Conclusion:Both of the miR-146a and CA have effects on synovial inflammation in OA，especially when mimics and CA were combined，the inhibition of inflammation was best.It could provide an experimental basis to clarify molecular mechanism，identify and develop novel drug targets of OA.