| 引用本文:杨新蕊1，缪希红2，冯晓静2，白云鹤1，田甜1.佛香饮治疗原发性痛经作用实验研究[J].世界中医药,2017,(10):. |
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| 佛香饮治疗原发性痛经作用实验研究 |
| Experimental Study on Effects of Foxiang Decoction in Treating Primary Dysmenorrhea |
| 投稿时间：2016-11-13 |
| DOI：10.3969/j.issn.1673-7202.2017.10.035 |
| 中文关键词: 佛香饮 原发性痛经 镇痛 子宫平滑肌 前列腺素F2α |
| English Keywords:Foxiang decoction Primary dysmenorrhea Analgesic Uterine smooth muscle PGF2α |
| 基金项目:河北省中医药管理局(2016159) |
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| 中文摘要: |
| 目的:观察佛香饮对原发性痛经的治疗作用,为其临床应用提供依据。方法:采用55 ℃和48 ℃热刺激建立小鼠疼痛模型、缩宫素法建立大鼠原发性痛经模型,观察佛香饮对痛经的镇痛作用;以痛经模型大鼠离体子宫平滑肌收缩幅度、频率为指标,评价佛香饮的镇痛作用及对子宫平滑肌活力的影响;以缩宫素致正常大鼠离体子宫平滑肌痉挛性收缩,观察佛香饮拮抗缩宫素的致痉作用;以痛经模型大鼠血清中前列腺素F2α(PGF2α)含量为指标,分析其镇痛作用机制。结果:55 ℃热板实验,佛香饮7.2 g/kg(按生药计)组小鼠痛阈低于模型组(P<0.05);48 ℃热板实验,佛香饮1.8、3.6和7.2 g/kg组痛阈均低于模型组(P<0.05或P<0.01);痛经模型大鼠扭体反应次数均低于模型对照组(P<0.05或P<0.01);佛香饮3.6和7.2 g/kg剂量组子宫平滑肌收缩频率显著低于模型组(P<0.05或P<0.01),收缩幅度显著高于模型组(P<0.05或P<0.01);佛香饮可剂量依赖性的降低缩宫素引起大鼠离体子宫平滑肌收缩曲线的上升高度;各剂量组血清中PGF2α的含量显著低于模型组(P<0.05或P<0.01)。结论:佛香饮对热刺激性疼痛及原发性痛经性疼痛均有显著的镇痛及抑制子宫平滑肌痉挛性收缩的作用,抑制PGF2α的合成或释放是其治疗痛经的机制之一。 |
| English Summary: |
| To investigate the effects of Foxiang decoction (FXD) in treating primary dysmenorrhea for providing the basis for clinical application.Methods:The mouse model of pain were established by using 55 ℃ and 48 ℃ thermal stimulation; and the rat model of primary dysmenorrhea was established by subcutaneous injection of oxytocin.The analgesic effect of Foxiang decoction on dysmenorrhea was observed by the two models.The contraction frequency and contraction amplitude of uterine smooth muscle were examined to evaluate the analgesic effect of FXD on primary dysmenorrhea model rats and its effects on the tension of uterus smooth muscle.As well as，it was examined that the antagonism effect of FXD on the uterine smooth of normal rats tetanic contraction induced by oxytocin.The contents of prostaglandin F2 (PGF2) in serum of rats with dysmenorrhea were determined to analyze the mechanism of its analgesic effect.Results:In the 55 ℃ hot plate test，the pain threshold of FXD7.2 g/kg group (dollars by crude drug) was lower than the model group (P<0.05); in the 48 ℃ hot plate test，the pain threshold of FXD 1.8，3.6 and 7.2 g/kg group were lower than the model group (P<0.05 or P<0.01); reaction times writhing dysmenorrhea rats were lower than the model group (P<0.05 or P<0.01); writhing responses number of rats in all FXD group were lower than that of model group (P<0.05 or P<0.01).In 3.6 and 7.2 g/kg of FXD dose group，the uterine smooth muscle contraction frequency was significantly lower than that of dysmenorrhea model group (P<0.05 or P<0.01)，but contraction amplitude was significantly higher than that of dysmenorrhea model group (P<0.05 or P<0.01).In uterine smooth muscle of normal rats，FXD significantly inhibited heighten of the contraction curve baseline induced by oxytocin by dose dependently.The content of PGF2 in the serum in all dose group was significantly lower than that of the MC group (P<0.05 or P<0.01).Conclusion:FXD has significant analgesic effect on heat pain and primary dysmenorrhea pain，and the inhibition of uterine smooth muscle spastic contraction pain.The inhibition of the synthesis or release of PGF2α is one of the mechanisms of its treatment of dysmenorrhea. |
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