世界中医药

目的：观察洋参御唐丸对大鼠血糖水平、胰岛结构及功能的影响。方法：将30只OLETF大鼠根据随机数字表法分为观察组和对照组，每组15只；15只同系非糖尿病的LETO大鼠为正常对照组。所有大鼠均为8周龄。正常对照组：在SPF级条件下标准饲料，单笼饲养；对照组：在正常对照组的基础上以2 mL蒸馏水灌胃；观察组：洋参御唐丸溶于蒸馏水中灌胃，疗程24周。治疗前后每组处死大鼠，进行血清葡萄糖浓度检测、采用酶联免疫吸附测定法（ELISA法）检测空腹胰岛素(FINS)；并通过HE染色和Masson复合染色观察胰岛结构的形态学观察。结果：1)胰腺HE染色形态学显示治疗后对照组胰岛形状不规则，出现大量的纤维组织增生，胰岛有明显的萎缩；观察组胰岛无明显肥大或萎缩，纤维组织不明显。2)胰腺Masson染色形态学显示治疗后，对照组发现大量纤维组织长入胰岛，与外分泌腺界限不明；而观察组仅见胰岛周边有少量纤维组织，未见纤维组织长入胰岛。3)治疗后，与正常对照组比较，对照组和观察组明显升高（P<0.05），ISI降低（P<0.05）。观察组FBG、FINS均低于对照组（P<0.05），ISI高于对照组（P<0.05）。结论:洋参御唐丸能有效调节OLETF大鼠的血糖水平和改善胰岛的功能和结构。

English Summary:

To observe the effect of Yangshen Yutang Pill on blood glucose, structure and function of pancreas in rats.Methods:Thirty 8-week-old OLETF rats were randomly divided into intervention group and control group with 15 rats in each group and 15 8-week-old syngeneic non-diabetic LETO rats were taken as blank control group. Blank control group: rats were fed at the SPF level with standard feedings and one rat in single cage; control group: 2 mL of distilled water was added compared to the blank control group; the intervention group: Rats took Yangshen Yutang Pill distilled into solution by gavage for 24 weeks. The rats were executed in each group before and after the treatment to test the serum glucose concentration and the fasting insulin (FINS) by enzyme-linked immunosorbent assay (ELISA method) and HE staining and Masson staining were used to observe the morphology of pancreas structure.Results:1) Pancreatic morphology by HE staining showed that the pancreas of rats in the control group after the treatment was irregular in shape with the emergence of a large number of fibrous tissue hyperplasia and there was obvious atrophy in the pancreas but no obvious hypertrophy or atrophy and fibrous tissue in the pancreas of rats in the intervention group. 2) Pancreatic morphology by Masson staining showed that a large number of fibrous tissue appeared in pancreas of rats in the control group and boundaries between pancreas and exocrine glands were ambiguous after the treatment, while only a small amount of fibrous tissue appeared surrounding the pancreas of rats in the intervention group with no fibrous tissue in the pancreas. 3) Compared with the blank control group, the control group and the intervention group were significantly higher after the treatment (P<0.05) and ISI decreased(P<0.05). The FBG and FINS in the intervention group were lower than those in the control group (P<0.05) and ISI was higher than that in the control group (P<0.05).Conclusion:Yangshen Yutang Pill may effectively regulate the blood glucose level of OLETF rats and improve the function and structure of pancreas.

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