| 引用本文:邱宏聪1，陈昭2,3，柴玲1，刘布鸣1.高效液相色谱法同时测定不同来源水翁花中的2种活性成分[J].世界中医药,2017,(10):. |
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| 高效液相色谱法同时测定不同来源水翁花中的2种活性成分 |
| Simultaneous Determination of Two Active Components in Flos Cleistocalycis Operculati by High Performance Liquid Chromatography |
| 投稿时间：2016-08-01 |
| DOI：10.3969/j.issn.1673-7202.2017.10.050 |
| 中文关键词: 水翁花 高效液相色谱法 活性成分 |
| English Keywords:Flos Cleistocalycis Operculati High performance liquid chromatography Active components |
| 基金项目:广西中医药研究院系统研究课题(桂中重自201401） |
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| 摘要点击次数: 1049 |
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| 中文摘要: |
| 目的：对水翁花中的熊果苷和2',4'-二羟基-6'-甲氧基-3'-5'-二甲基查耳酮进行含量测定研究，建立药材的质量标准。方法：采用高效液相色谱（HPLC）法：以Diamonsil-C18(5 μm，250×4.6 mm)为色谱柱，以乙腈-0.05%磷酸(60:40)为流动相进行梯度洗脱，检测波长254 nm，流速1.0 mL/min，柱温25 ℃；结果：采用HPLC法建立了8批药材的指标性成分指纹图谱并进行准确定量；结论：该方法快速、高效、准确，能够全面、有针对性地对水翁花药材的质量优劣进行评价。 |
| English Summary: |
| To evaluate the quality of Flos Cleistocalycis Operculati by quantitatively determining arbutin and 2',4'-dihydroxy-6'-methoxy-3'-5'-dimethylchalcone. Methods:HPLC was used for determination, with Diamonsil-C18 (5 μm,250×4.6 mm) column and a mobile phase containing acetonitrile-0.05% phosphoric acid; the detection wavelength was 254 nm, column temperature 25 ℃ and the elution mode was gradient, with a flow rate of 1.0 mL/min; Results:Eight batches of herb samples were accurately determined and established fingerprints. Conclusion:The proposed method was fast, efficient and accurate, which can be used in quality evaluation of Flos Cleistocalycis Operculati comprehensively and specifically. |
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