To evaluate the quality of Flos Cleistocalycis Operculati by quantitatively determining arbutin and 2',4'-dihydroxy-6'-methoxy-3'-5'-dimethylchalcone. Methods:HPLC was used for determination, with Diamonsil-C18 (5 μm,250×4.6 mm) column and a mobile phase containing acetonitrile-0.05% phosphoric acid; the detection wavelength was 254 nm, column temperature 25 ℃ and the elution mode was gradient, with a flow rate of 1.0 mL/min; Results:Eight batches of herb samples were accurately determined and established fingerprints. Conclusion:The proposed method was fast, efficient and accurate, which can be used in quality evaluation of Flos Cleistocalycis Operculati comprehensively and specifically.