| 引用本文:林久茂1，2，李琼瑜1，严兆坤1，赖子君1，靳祎祎1，彭军1，2.白花蛇舌草对大肠癌耐药细胞microRNAs表达的影响[J].世界中医药,2017,(11):. |
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| 白花蛇舌草对大肠癌耐药细胞microRNAs表达的影响 |
| Effects of Herba Hedyotis Diffusae on Expression of microRNAs in Colorectal Cancer Drug Resistant Cells |
| 投稿时间：2016-08-10 |
| DOI：10.3969/j.issn.1673-7202.2017.11.052 |
| 中文关键词: 白花蛇舌草 大肠癌 microRNA 耐药 |
| English Keywords:Herba Hedyotis Diffusae Colorectal cancer microRNA Drug resistance |
| 基金项目:教育部博士点基金博导类项目（20133519110003）;陈可冀中西医结合发展基金项目（CKJ2015007，CKJ2014013） |
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| 中文摘要: |
| 目的：探讨白花蛇舌草（Hedyotic Diffusa Wilid，HDW）对大肠癌5-FU耐药细胞miRNAs表达的调控作用，进一步揭示其逆转大肠癌多药耐药（Multidrug Resistance，MDR）作用机制。方法：通过体外培养HCT-8/5-FU细胞，经HDW处理，采用MTT检测细胞活力、倒置显微镜观察细胞形态、microRNA（miRNA）表达谱芯片分析、QPCR验证差异miRNA表达、GO及Pathway分析预测差异miRNA调控的信号通路。结果：HDW可显著抑制大肠癌HCT-8/5-FU耐药细胞的活力，降低细胞密度；HDW调控57个miRNA差异表达，其中上调23个，下调34个；QPCR验证HDW上调miR-92b-5p、miR-1247-3p、miR-4800-5p的表达，下调miR-4454、miR-4443、miR-483-5p的表达；信号通路预测显示HDW可调控PI3K/Akt、TGF-β/Smad、MAPK、P53、Wnt、JAK-STAT等多条与细胞耐药、迁移、侵袭、增殖、凋亡有关的信号通路。结论：HDW对大肠癌5-FU耐药细胞HCT-8/5-FU具有显著的抑制作用，通过调控miRNAs表达是其逆转大肠癌耐药的作用机制之一。 |
| English Summary: |
| To study the regulatory effect of Herba Hedyotis Diffusae on multidrug-resistance (MDR)-related microRNAs (miRNAs) in colorectal cancer，and further explore the underlying mechanisms of its MDR reversion.Methods:The signal pathway regulated by miRNA was forecasted by MTT assays，morphology observation，miRNA array analysis，QPCR validation，GO and Pathway analysis through treatment of HDW in HCT-8/5-FU cells.Results:HDW significantly inhibited colorectal cell viability and decreased density of HCT-8/5-FU cells.There were 23 up-regulated and 34 down-regulated in 57 miRNA differential expression after HDW treatment; and QPCR verification confirmed that HDW increased expression of miR-92b-5p，miR-1247-3p and miR-4800-5p and decreased expression of miR-4454，miR-4443，miR-483-5p; and the signal pathway prediction showed that HDW could control PI3K/Akt，TGF-β/Smad，MAPK，P53，Wnt and JAK-STAT signaling pathways regulating MDR，migration，invasion，proliferation and apoptosis.Conclusion:HDW can remarkably suppress proliferation in HCT-8/5-FU cells，and it is one of the mechanisms to reverse the drug resistance of colorectal cancer by regulating miRNAs expression. |
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