| 引用本文:张亚丽，高简，苗祥贞，张潇，刘永刚，谭鹏.黄连多糖中单糖组成的HPLC-MSn法快速识别[J].世界中医药,2017,(11):. |
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| 黄连多糖中单糖组成的HPLC-MSn法快速识别 |
| Monosaccharide Composition Analysis of Polysaccharides from Rhizoma Coptidis Based on HPLC-MSn |
| 投稿时间：2016-12-28 |
| DOI：10.3969/j.issn.1673-7202.2017.11.053 |
| 中文关键词: 黄连多糖 PMP 单糖 HPLC-MSn |
| English Keywords:Rhizoma Coptidis polysaccharide PMP Monosaccharide HPLC-MSn |
| 基金项目:青年科学基金项目（81102807） |
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| 中文摘要: |
| 目的：探讨采用HPLC-MSn法快速识别黄连多糖中的单糖组成。方法：黄连药材经过提取、分离、除蛋白、除色素，最终得到黄连多糖，用2 mol/L的三氟乙酸水解黄连多糖，以1-苯基-3-甲基-5-吡唑啉酮(PMP)作单糖衍生化试剂，利用HPLC-MSn识别黄连中单糖的组成，并解析其衍生物的裂解规律。结果：通过分析，得到的黄连多糖中含有甘露糖、核糖、鼠李糖、半乳糖醛酸、葡萄糖、半乳糖、阿拉伯糖/木糖、岩藻糖，其中核糖、岩藻糖为首次在黄连多糖中报道的单糖。结论：HPLC-MSn法对中药多糖组分中的单糖成分进行分析具有重要的指导意义。 |
| English Summary: |
| To explore a rapid identification of monosaccharide composition of polysaccharide from Rhizoma Coptidis through HPLC-MSn. Methods:After being extracted, separated, removed of protein and pigment, Rhizoma Coptidis polysaccharide was obtained from Rhizoma Coptidis. Polysaccharides of Rhizoma Coptidis was hydrolyzed with the TFA of 2 mol.L-1. 1-phenyl-3-methyl-5-pyrazolone (PMP) was used as derivatization reagent. And high performance liquid chromatography-mass spectrometry (HPLC-MSn) was used to identify the composition of monosaccharide, and the resolve fragmentation regularity of its derivatives was analyzed. Results:Through analysis, the monosaccharides of polysaccharides from Rhizoma Coptidis were mannose, ribose, rhamnose, galacturonic acid, glucose, galactose, arabinose/ xylose, fucose, among which ribose and fucose were the first reported monosaccharides that obtained from polysaccharide of Rhizoma Coptidis. Conclusion:HPLC-MSn has a guiding significance in analyzing the monosaccharide composition of polysaccharides from Chinese materia medica. |
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