世界中医药

作者	单位
黄娟1，张靖1，宫璐1，李西文2，张鹏2，徐文1，丘小惠1，黄志海1	1 广州中医药大学第二附属医院，广东省中医药科学院中国中医科学院广东分院，广州,510006 2 中国中医科学院中药研究所，北京，100700

摘要点击次数: 1059

全文下载次数: 910

中文摘要:

目的：探讨精准煮散饮片与市售原饮片成分溶出及质量均一性差异。方法：应用ITS2序列作为DNA条形码对枳壳饮片进行鉴定；比较枳壳市售原饮片及精准煮散饮片的干膏收率；采用HPLC-DAD法进行3批次饮片混合粉碎前后质量均一性、指纹图谱及相似度评价。结果：枳壳煮散饮片出膏率略高于市售饮片出膏率；精准煮散饮片提取液中柚皮苷溶出较市售饮片高，市售饮片柚皮苷、新橙皮苷批间溶出量有明显的差异性RSD分别为9.71%及24.71%，混合制成煮散饮片后差异缩小RSD分别为4.73%、4.94%。指纹图谱相似度评价结果显示，制成煮散饮片后相似度提高，且共有峰峰面积均有提高。结论：枳壳市售饮片制成精准煮散饮片基本属性没有变化，但精准煮散饮片能提饮片的煎煮效率及均一性，发展精准煮散饮片具有良好的应用前景。

English Summary:

This study aimed to evaluate the composition dissolution and quality uniformity of precise powder decoction pieces (PPDP) of Aurantii Fructus (AF),compared with the traditional commercial slices.Chemical fingerprint methods and DNA molecular identification technology based on internal transcribed spacer 2 (ITS2) were applied.Methods:Different specifications of PPDP were prepared,their dry extract contents were compared with those of commercial slices.Three batches of commercial slices were collected,and the content uniformity,fingerprint and similarity evaluation before and after the mixing and pulverization were analyzed by HPLC-DAD and DNA sequence alignment.Results:Paste rate of PPDP were slightly higher than that of the traditional commercial slices.The dissolution of naringin of PPDP was higher than that of traditional commercial slices.RSD of inter-assay dissolutions of naringin and neohesperidin of commercial slices were 9.71% and 24.71% respectively,which could be reduced to 4.73% and 4.94% after mixing and preparing into PPDP.Finger print image shows there were high similarity between decoction and slices.Conclusion:PPDP and traditional commercial slices of AF shared consistent properties.PPDP apparently improved the extraction rate,the dissolution rate and quality uniformity,and it is recommended to be used in clinical practice.

查看全文查看/发表评论下载PDF阅读器