| 引用本文:张方博1，许淼2，张毅1，李德凤1，赵海誉1，杨洪军1，边宝林1.黄连解毒汤对大鼠上火牙龈炎的治疗作用[J].世界中医药,2017,(12):. |
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| 黄连解毒汤对大鼠上火牙龈炎的治疗作用 |
| Effects of Huanglian Jiedu Decoction in Rat Shanghuo Gingivitis |
| 投稿时间：2017-11-03 |
| DOI：10.3969/j.issn.1673-7202.2017.12.004 |
| 中文关键词: 黄连解毒汤 上火 牙龈炎 糖酵解 能量代谢 氧化应激 |
| English Keywords:Huanlian jiedu decoration Shanghuo Gingivitis Glycolysis Energy metabolism Oxidative stress |
| 基金项目:国家重点基础研究发展计划（973计划）项目（2014CB543003）；国家自然科学基金青年基金（81403207） |
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| 中文摘要: |
| 目的：采用牙龈黏膜下注射内毒素法构建大鼠上火牙龈炎模型，观察黄连解毒汤对大鼠上火牙龈炎的治疗作用。方法：雄性SD大鼠随机分为7组：正常对照组、溶剂对照组、上火牙龈炎模型组、黄连解毒汤低（0.25 g/kg）、中（0.50 g/kg）、高剂量组（1.00 g/kg）和甲硝唑芬布芬胶囊组。代谢笼喂养观测饮水量和尿量；LC-ESI-MS/MS法检测牙龈组织蛋白表达；生化试剂盒结合酶标仪检测牙龈组织氧化应激相关分子总超氧化物歧化酶（Total Superoxide Dismutase，T-SOD）和过氧化氢酶（Hydrogen Peroxidase，Catalase，CAT）的表达；Western Blotting法检测牙龈组织能量代谢相关信号通路TSC的表达。结果：上火牙龈炎时，大鼠饮水量和尿量减少，T-SOD和CAT表达水平降低，果糖二磷酸醛缩酶C、磷酸甘油醛异构酶1和TSC2均表达升高。黄连解毒汤可增加饮水量和尿量，升高T-SOD和CAT的水平，降低果糖二磷酸醛缩酶C和磷酸甘油醛异构酶1的表达，抑制能量代谢相关通路分子TSC2的表达。结论：黄连解毒汤可通过TSC信号通路和糖酵解相关分子抑制能量代谢，并抑制氧化应激，发挥治疗上火牙龈炎的作用。 |
| English Summary: |
| To evaluate the effects of huanglianjiedu decoration (HLJD) on the treatment of rat Shanghuo gingivitis by injecting lipopolysaccharide (LPS) in tooth-supporting tissues. Methods:Gingival inflammation was established by LPS injection. Adult male rats were divided into seven equal groups:control group (no treatment group), negative control group (sterile saline was injected into gingival tissue followed by oral gavage with saline), positive control group (LPS injection was followed by oral gavage with saline), HLID low-dose, middle-dose, high-dose group (LPS injection was followed by oral gavage with HLJD); metronidazole and fenbufencapsule (MFC) group (Oral gavage with MFC was followed by LPS injection). After 3 days, the rats were sacrificed and gingival tissue samples were randomly evaluated by biochemistry method, LC-ESI-MS/MS and western blotting. Results:The levels of water intake, urine volume, total superoxide dismutase (T-SOD) and hydrogen peroxidase (catalase, CAT) in the model group were apparently lower than those in the control group. Meanwhile, fructose-bisphosphate aldolase C, triosephosphate isomerase 1 and TSC2 in the model group were significantly increased than those in the control group. HLJD could reverse those effects. ANOVA was used for the statistical analyses, with P<0.05 regarded as significant. Conclusion:HLJD treatment is effective in reducing energy metabolism and oxidative stress in induced rat gingivitis, resulting in a decreased level of fewer destructive effects. |
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