To evaluate the effects of huanglianjiedu decoration (HLJD) on the treatment of rat Shanghuo gingivitis by injecting lipopolysaccharide (LPS) in tooth-supporting tissues. Methods:Gingival inflammation was established by LPS injection. Adult male rats were divided into seven equal groups:control group (no treatment group), negative control group (sterile saline was injected into gingival tissue followed by oral gavage with saline), positive control group (LPS injection was followed by oral gavage with saline), HLID low-dose, middle-dose, high-dose group (LPS injection was followed by oral gavage with HLJD); metronidazole and fenbufencapsule (MFC) group (Oral gavage with MFC was followed by LPS injection). After 3 days, the rats were sacrificed and gingival tissue samples were randomly evaluated by biochemistry method, LC-ESI-MS/MS and western blotting. Results:The levels of water intake, urine volume, total superoxide dismutase (T-SOD) and hydrogen peroxidase (catalase, CAT) in the model group were apparently lower than those in the control group. Meanwhile, fructose-bisphosphate aldolase C, triosephosphate isomerase 1 and TSC2 in the model group were significantly increased than those in the control group. HLJD could reverse those effects. ANOVA was used for the statistical analyses, with P<0.05 regarded as significant. Conclusion:HLJD treatment is effective in reducing energy metabolism and oxidative stress in induced rat gingivitis, resulting in a decreased level of fewer destructive effects.