| 引用本文:汪琴静1，徐莉2，包洁2，季巾君2，谢冠群2，范永升2.“上火”人群中Activin A、E-selectin的差异表达研究[J].世界中医药,2017,(12):. |
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| “上火”人群中Activin A、E-selectin的差异表达研究 |
| Research on the Differential Expression of Activin A and E-selectin in the Population of Excessive Internal Heat |
| 投稿时间：2017-11-03 |
| DOI：10.3969/j.issn.1673-7202.2017.12.005 |
| 中文关键词: 上火 激活素A E选择素 炎性反应 |
| English Keywords:Shanghuo Activin A E selectin Inflammatory reaction |
| 基金项目:国家重点基础研究发展计划（973计划）项目（2014CB543001，2014CB543002）；国家自然科学基金项目（81673857） |
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| 中文摘要: |
| 目的：通过对“上火”人群中细胞因子激活素A（ActivinA）及E选择素（E-selectin）血清含量的测定以及外周血单个核细胞基因表达水平检测，探讨激活素A及E选择素在“上火”人群发病机制的影响和作用。方法：采集临床患者血液样本并提取血清及外周血单个核细胞。应用酶联免疫吸附试验（ELISA）测定血清中激活素A及E选择素的含量，应用RT-PCR法检测激活素A及E选择素的基因表达水平。结果：“上火”患者血清中activinA、E-selectin的水平以及外周血单个核细胞activinA、E-selectin基因表达水平高于正常平和质人群；观察组外周血中activinA、E-selectin的基因表达水平高于阴虚“上火”人群。结论：“上火”是一种局部的炎性反应，机体内环境在多种因素的作用下激活素A水平升高，影响炎性反应介质的作用或者本身作为一种炎性反应介质活化血管内皮细胞刺激E选择素的分泌与合成，诱导炎性反应，这是“上火”机制之一。 |
| English Summary: |
| To study of activin A and E selectin in the pathogenesis of “shanghuo” people and influence effect through the determination of serum content and the level of gene expression in peripheral blood mononuclear cells of activin A or E-selectin who suffered from excessive internal heat. Methods:Clinical lit crowd and gentleness in blood samples was collected and serum and peripheral blood mononuclear cells from blood samples was extracted. By enzyme-linked immune sorbent adsorption assay, the content of serum activin A and E-selectin were determined, and RT-PCR detection was sued to detect gene expression level of activin A and E-selectin. Results:The level of activin A and E-selectin in serum of patients with excessive internal heat was higher than that of normal and healthy people. The level of activin A and E-selectin gene expression in peripheral blood mononuclear cells of patients with internal heat was higher than that of normal and healthy people. The gene expression level of activin A and E-selectin in peripheral blood was higher than that of yin deficiency and heat syndrome. Conclusion:Shanghuo is a local inflammation. Under stress, activin A levels are elevated, effecting inflammatory mediators or itself as a mediator of inflammation activated vascular endothelial cells stimulate E-selectin secretion and synthesis, induced inflammatory reaction and to cause the disorder of lipid metabolism in the body's physiological balance, causing Shanghuo. |
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