| 引用本文:毛连根1，刘昌铭1，杨粟1，江婷婷2，陈钟梁1，屠慧惠1，陈静1，胡玉婷2，甘霖2，李仲杰1，李继承1，2.知柏地黄丸对阴虚“上火”证血清抗炎凝血蛋白的影响[J].世界中医药,2017,(12):. |
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| 知柏地黄丸对阴虚“上火”证血清抗炎凝血蛋白的影响 |
| Effect of Zhibai Dihuang Granule on Anti-inflammatory and Clotting Serum Proteins in Yin-deficiency Shanghuo Syndrome |
| 投稿时间：2017-11-03 |
| DOI：10.3969/j.issn.1673-7202.2017.12.006 |
| 中文关键词: 知柏地黄丸 阴虚“上火” 蛋白组学 抗炎 凝血 发病机制 |
| English Keywords:Zhibai Dihuang Granule Yin-deficiency Shanghuo syndrome Proteomics Anti-inflammatory Clotting Pathogenesis |
| 基金项目:国家重点基础研究发展计划（973计划）项目（2014CB543002） |
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| 中文摘要: |
| 目的：探究知柏地黄丸（Zhibai Dihuang Granule；ZDG）对阴虚“上火”证血清抗炎凝血蛋白的影响。方法：通过腹腔注射三碘甲状腺原氨酸（Triiodothyronine；T3）处理SD大鼠建立阴虚模型。在该模型基础上往大鼠牙龈出滴加牙龈卟啉单胞菌叠加口腔牙龈炎模型，建立阴虚“上火”证大鼠模型。用滋阴降火药知柏地黄丸对大鼠进行治疗，以iTRAQ-2DLC-MS/MS蛋白组学技术与生物信息学手段分析治疗前后阴虚“上火”大鼠血清差异性蛋白表达谱，探索知柏地黄丸治疗阴虚“上火”的生物学机制。结果：知柏地黄丸观察组大鼠血清凝溶胶蛋白（Gsn）、纤溶酶原（Plg）、纤维蛋白原α链（Fga）、胸腺素β4（Tmsb4x）比值显著上调（Fold Change>1.25）；胶原蛋白α2-I型链（Col1a2）比值显著下调（Fold Change<0.8）。Gsn是机动蛋白丝的切割蛋白，参与细胞凋亡、清除损伤组织、调节炎性介质等生物学功能；Plg是血液纤维蛋白水解酶无活性的前体，参与机体纤溶、细胞迁移、细胞外基质降解等多种生物过程；Fga是由肝脏合成的纤维蛋白前体，能在内源凝血因子或外源组织因子作用下生成纤维蛋白，参与机体的凝血过程；Colla2是由肝、巨核细胞合成的凝血蛋白酶，是活化Fga的凝血稳定因子，Fga在Colla2的作用下参与完成凝血过程；T-β4是由胸腺产生的淋巴生长因子，具有通过抑制巨噬细胞的迁移、增强抗原提呈细胞功能、抑制炎性因子、促进组织重塑、血管生成和伤口愈合修复等生物学功能。结论：知柏地黄丸通过对Gsn、Plg、Fga、Colla2、T-β4水平的调节，清除损伤组织，释放肌动蛋白、细菌脂多糖，抑制细胞凋亡作用；并且可以调节炎性因子，改变炎性反应部位血液流变动力学障碍，促进炎性反应损伤的愈合的作用。 |
| English Summary: |
| To explore the influence of Zhibai Dihuang Granule (ZDG) on the anti-inflammatory clotting serum proteins in Yin-deficiency Shanghuo(YDS) syndrome. Yin-deficiency SD rats were induced by intraperitoneal injection of Triiodothyronine (T3). The models of gingivitis in rats were induced by dropwise add of porphyromonas gingivalis model. YDS syndrome rats were treated with ZDG for 1 week. iTRAQ-2DLC-MS/MS was used in screening and identifying the serum differential proteins. With the treatment of ZDG, the serum level of Gelsolin (Gsn), Plasminogen (Plg), Fibnnogenalpha chain (Fga), and Thymus beta-4 (T-β4) were significantly increased (fold change>1.25), while the serum level of Collagulation factor XIII Achain and Colla2 were significantly decreased (fold change<0.8). Gsn possesses the function of removing damaged tissue, releasing the actin, inhibiting the apoptosis of the cells, and inhibiting the apoptosis of the cells. Colla2 and Plgcan regulated the change of blood rheology in the inflammatory site. Gsn and T-beta 4 presented the ability in regulating inflammatory cytokines to promote the healing of inflammatory damage. Conclusion:ZDG may play an important role in clearing actin and lipopolysaccharide released by damage tissue and inhibiting the cell apoptosis. Besides, ZDG may regulate inflammatory factors, change the blood rheology of the inflammatory site, promot the healing of inflammatory damage. In short, ZDG treats YDH syndrome by regulating these proteins associated with anti-inflammatory and clotting. |
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