To analyze and study the chemical constituents of Radix Astragali seu Hedysari before and after compatibility of Radix Astragali seu Hedysari and Rhizoma Atractylodis Macrocephalae based on HPLC, and to explain the scientific connotation of the rational compatibility of Chinese herbal medicine. Methods:The linear relationship analysis was conducted by HPLC and the sample recovery test was used to investigate the preciseness of HPLC. HPLC fingerprint:astragaloside reference solution, negative reference solution lack of Radix Astragali seu Hedysari, Radix Astragali seu Hedysari test solution and the compatibility of Radix Astragali seu Hedysari and Rhizoma Atractylodis Macrocephalae test solution for HPLC fingerprint analysis, and chemical constituents of Radix Astragali seu Hedysari and Rhizoma Atractylodis Macrocephalae from 3 areas were analyzed. Results:1) The linear relationship was good in the range of 1.024 ~ 10.24 μg, and with 2.2 sample recovery test results. 2) The average recovery rate was 99.60%, and RSD was 2%, which showed that the sample recovery rate was good, and the preciseness of HPLC was high. 3) HPLC fingerprint analysis showed that in reference solution, negative reference solution lack of Radix Astragali seu Hedysari, Radix Astragali seu Hedysari test solution and the compatibility of Radix Astragali seu Hedysari and Rhizoma Atractylodis Macrocephalae test solution, no interference in negative reference solution lack of Radix Astragali seu Hedysari and the baseline separation of astragaloside and other components were good. 4) Analysis in 3 ares showed that the content of astragaloside increased with the compatibility of Radix Astragali seu Hedysari and Rhizoma Atractylodis Macrocephalae, among which that content was the highest from Inner Mongolia. Conclusion:The main chemical constituents of Radix Astragali seu Hedysari after the compatibility of Radix Astragali seu Hedysari and Rhizoma Atractylodis Macrocephalae, among which the content in Inner Mongolia is the highest.