| 引用本文:孟红旭，姚明江，任钧国，刘建勋.延胡索叔胺碱和季胺碱对豚鼠心室肌细胞动作电位和HERG通道电流的影响[J].世界中医药,2018,(01):. |
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| 延胡索叔胺碱和季胺碱对豚鼠心室肌细胞动作电位和HERG通道电流的影响 |
| Effects of Tertiary Alkaloids and Quaternary Alkaloids of Rhizoma Corydalis on Action Potential in Guinea Pig Ventricular Myocytes and HERG Channel Currents |
| 投稿时间：2017-12-20 |
| DOI：10.3969/j.issn.1673-7202.2018.01.001 |
| 中文关键词: 延胡索 叔胺碱 季胺碱 动作电位 HERG通道 |
| English Keywords:Corydalis Tertiary alkaloids Quaternary alkaloids Action potential HERG channel |
| 基金项目:国家重点基础研究发展计划(973计划)项目（2015CB554405）；中国中医科学院创新团队建设项目（YS1303）；中国中医科学院自助选题项目（zz070821） |
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| 中文摘要: |
| 目的：观察延胡索叔胺碱和季胺碱对动作电位和HERG通道的影响，探讨2种生物碱抗心律失常的作用机制。方法：采用全细胞膜片钳技术记录急性分离的豚鼠心室肌细胞动作电位和培养的稳定转染HERG通道蛋白的HEK293细胞的HERG通道电流。结果：叔胺碱（30,100 mg/L）可延长APD90（90%复极化时程）；1 mg/L和3 mg/L季胺碱可缩短APD90，加深静息电位；30 mg/L和100 mg/L季胺碱可延长APD90；叔胺碱（3～300 mg/L）可浓度依赖性抑制HERG尾电流幅度，IC50为55 μmol/L;1 mg/L和3 mg/L季胺碱可明显升高HERG尾电流电流幅度，而30 mg/L和100 mg/L季胺碱可明显降低尾电流幅度。结论：延胡索叔胺碱可浓度依赖性延长动作电位时程和抑制HERG通道电流，而季胺碱高浓度与叔胺碱作用相同，低浓度则能够缩短动作电位时程和兴奋HERG通道电流，呈现双相性。 |
| English Summary: |
| To investigate the effects of tertiary alkaloids and quaternary alkaloids of Rhizoma Corydalis on action potential and HERG channel, and to explore the mechanism of two alkaloids in anti-irrhythmia. Methods: Whole cell patch clamp technique was used to record the action potential of acutely isolated guinea pig ventricular myocytes and the HERG channel currents of cultured HEK293 cells stably transfected with HERG channel protein. Results: Tertiary alkaloids (30, 100 mg/L) could prolong APD90, and quaternary alkaloids (1, 3 mg/L) could shorten APD90 and enhance the resting potential. Quaternary alkaloids (30, 100 mg/L) prolonged APD90. Tertiary alkaloids (3-300 mg/L) inhibited HERG tail current amplitude in concentration-dependent manner. IC50 was 55 mg/L; quaternary alkaloids (1, 3 mg/L) could significantly increase HERG tail current amplitude, and quaternary alkaloids (30, 100 mg/L) could reduce the tail current amplitude. Conclusion:Corydalis tertiary alkaloids prolonged APD and inhibited HERG currents in concentration-dependent manner. Quaternary alkaloids in high concentration have the same effect as tertiary alkaloids and quaternary alkaloids in low concentration decrease APD and excite HERG currents, showing a double phase. |
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