To study the protective effects and mechanisms of Yinxing Mihuan Oral Solution for cerebral microvascular endothelial cells (CMEC) and SH-SY5Y cells on inflammatory induced by oxygen/glucose deprivation (OGD) and reperfusion, as well as the effects of Yinxing Mihuan oral solution on autophagy pathways mediated by endothelial nitric oxide synthase (eNOS) depended by Beclin-1. Methods: Oxygen/glucose deprivation and oxygen/glucose reintroduction model was established in murine cerebral microvascular endothelial cells and SH-SY5Y cells by (OGD) and reperfusion. Meanwhile, different concentrations of Yinxing Mihuan Oral Solution (5.15 mg/mL, 2.575 mg/mL and 1.545 mg/mL) were used, and efficient compositions were intervened. Through the observation of the concentrations of inflammatory factors IL-1β, TNF-α and IL-6 in cell cultivated supernate and the excitation of autophagy pathways. Brain protective mechanism of Yinxing Mihuan active principles by apoptosis rate adopted the PI/ Annexin V double staining analysis was discussed. Results: Yinxing Mihuan Oral Solution (5.15 mg/mL, 2.575 mg/mL and 1.545 mg/mL) could restrain the contents of TNF-α, IL-1β and IL-6 in cell culture supernatant of CMEC. Yinxing Mihuan Oral Solution (2.575 mg/mL) significantly promoted the phosphorylation of eNOs and Yinxing Mihuan Oral Solution (2.575 mg/mL and 1.545 mg/mL) restrained the Caspase-3, LC3II and Beclin-1 expression. The effects of L-1β and IL-6 were significantly better than that of ginkgo biloba extract and mellea armillaria. Yinxing Mihuan Oral Solution (2.575 mg/mL and 1.545 mg/mL) also decreased SH-SY5Y cells apoptosis rate (Annexin V+/PI-cells), with significant difference. Conclusion: Yinxing Mihuan oral solution could promote the eNOs phosphorylation, which may be the interventional targets in restraining the cells' apoptosis and autophagy induced by OGD and reperfusion.