To investigate the effects of Yinxing Mihuan Oral Solution combined with Ginkgo Leaf Extracts and Armillariella Mellea Powders (GLE/AMP Oral Solution), a marketed drug for ischemic cardio-cerebrovascular diseases in rat models of multiple cerebral infarction (MCI). Methods: SD rats were randomly divided into sham group, model group, ginaton group (18 mg/kg), Extract of Ginkgo Biloba (EGB) group (9 mg/kg), armillaria mellea Tablets group (600 mg/kg), armillaria mellea Powder group (300 mg/kg), and Yinxing Mihuan Oral Solution groups (618 mg/kg and 309 mg/kg). The multiple cerebral infarction rat models were established by injection of fluorescent microsphere suspension serous into the direction of middle cerebral artery through arteria carotis communis. After intragastric administration for 28 days, the effects of each group were tested, including neurological function score, grasping force, concentration of serum TNF-α, level of amino acid neurotransmitters and pathomorphology in brain tissue were investigated. Results: Compared with the sham group, significant neurological function impairment was observed in the MCI model, with reduced grasping force, as well as higher level of Glutamate (Glu) and γ-aminobutyric acid (GABA) in the brain and TNF-α in the serum. Pathological observation showed brain damage with significant infarcts, disorganized neurons arrangement and inflammatory cell infiltration. The immunohistochemistry analysis indicated self-repair expression of CD34 and GFAP, and low expression of NeuN. After administration of Yinxing Mihuan Oral Solution for 28 days, the neurological function was improved, and the grasping force was increased. The level of Glu and GABA in the brain and TNF-α in the serum were decreased compared with those in the model group (P<0.05~0.01). Pathological observation showed that the Yinxing Mihuan Oral Solution could improve the degree of neurological function impairment, and the immunohistochemistry analysis indicated the Yinxing Mihuan Oral Solution could significantly promote the repair of cerebrovascular endothelial cell, glia and neuron. Besides, the effects of Yinxing Mihuan Oral Solution on improvement of neurological function and reducing the level of Glu and GABA in the brain were superior than that in the EGB group; the effect of Yinxing Mihuan Oral Solution in grasping force was superior than that in the Armillaria Mellea Powder group; and the effect of GLE/AMP Oral Solution in reducing the level of TNF-α in the serum was superior to that in the EGB group and Armillaria Mellea Powder group. Conclusion: The Yinxing Mihuan Oral Solution could protect cerebral ischemia and improve neurological function impairment, and combination use of Ginkgo Leaf Extracts and Armillariella Mellea Powders was superior in single component, represented certain synergistic interaction effect. |