| 引用本文:李磊1，徐立1，李振2，金龙1，葛争艳1，史跃1，马彦雷1，刘建勋1.芪龙胶囊对犬急性心肌缺血的影响[J].世界中医药,2018,(01):. |
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| 芪龙胶囊对犬急性心肌缺血的影响 |
| Effects of Qilong Capsule on Anesthetized Canine Experimental Myocardial Infarction |
| 投稿时间：2017-12-20 |
| DOI：10.3969/j.issn.1673-7202.2018.01.007 |
| 中文关键词: 急性心肌缺血 动物模型 犬 冠脉流量 芪龙胶囊 |
| English Keywords:Acute myocardial ischemia (AMI) Animal model Canine Flow of coronary artery Qilong capsule |
| 基金项目:国家重点基础研究发展计划(973计划)项目(2015CB554405)；国家科技重大专项“重大新药创制”（2017ZX09301018） |
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| 中文摘要: |
| 目的：观察芪龙胶囊对犬急性心肌缺血缺血、心肌梗死的保护作用。方法：采用犬冠脉前降支结扎的方法建立犬急性心肌缺血模型。36只犬随机分为6组，每组6只动物，分别为：模型对照组，合心爽组(0.5 mg/kg)，复方丹参片(0.24片/kg)组，芪龙胶囊256、128及64 mg/kg剂量组；采用心外膜电图标测心肌缺血范围及程度，定量组织学(N-BT染色法)测定心肌梗死范围，超声多普勒血流计测定心脏冠脉血流量，血气分析仪测定心肌耗氧量，全自动生化分析仪测定血清肌酸激酶(CK)、乳酸脱氢酶(LDH)的变化。结果：芪龙胶囊经十二指肠给药可明显减少N-BT所示的犬心肌梗死面积/心室面积的比率；明显减轻由心外膜电图标测的心肌缺血程度(药后15 min~180 min)，与模型组比较均有显著差异；可减小由心外膜电图标测的心肌缺血范围(药后15 min~180 min)；增加冠脉流量(药后30 min~45 min)；对心肌缺血及心肌梗死引起的血清CK、LDH活性升高有明显抑制作用，对急性心肌缺血后动脉、静脉血氧含量未见明显影响。结论：芪龙胶囊对犬急性心肌缺血模型表现出较好的心肌保护作用，可能与增加冠脉血流量，改善心肌供血有关。 |
| English Summary: |
| To observe the effects of Qilong capsule on acute myocardial ischemia (AMI) in the anesthetized dogs. Methods: Thirty-six dogs were randomized into six groups (n＝6), model group, Herbesser (0.5 mg/kg) group, Compound Danshen tablet (0.24 tablet/kg) group, Qilong capsule 256, 128, 64 mg/kg group. Acute myocardial ischemia model were established by ligating left anterior descending coronary arteries (LAD) of dogs. Changes of ST segment were determined by epicardial electrocardiogram (ECG), and coronary blood flow (CBF) were measured by ultrasonic Doppler flow meter. Myocardial oxygen consumption was measured by automatic blood gas analyzer, and serum creatine kinase (CK) and lactate dehydrogenase (LDH) were observed by fully automatic biochemical analyzer. Myocardial infarct size was assessed by nitro blue tetrazolium (N-BT) staining. Results: Administration of Qilong capsule reduced the myocardial infarct area/left ventricle area significantly compared with model group. Administration of Qilong capsule improved the ECG performance of Σ-ST from 15 min~180 min after the administration, and decreased the area of acute myocardial ischemia (N-ST) from 15 min~180 min after the administration. Qilong capsule can also increase blood flow of coronary artery from 30 min to 45 min after the administration. Compared with model group, Qilong capsule decreased serum CK and LDH significantly. Qilong capsule had no significant influence on blood oxygenation content. Conclusion: Qilong capsule protects myocardium in canine experimental myocardial infarction models, probably through proving blood supply of the cardiac muscle. |
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