| 引用本文:宋文婷1，刘建勋1，姚明江1，王光蕊1，王益民2，王勇2，徐立2.银杏蜜环口服溶液减轻脑缺血再灌注大鼠脑梗死的实验研究[J].世界中医药,2018,(01):. |
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| 银杏蜜环口服溶液减轻脑缺血再灌注大鼠脑梗死的实验研究 |
| Study on Yinxing Mihuan Oral Solution in Alleviating Ischemia/reperfusion Injury on Focal Cerebral Infarction Model Rats |
| 投稿时间：2017-12-20 |
| DOI：10.3969/j.issn.1673-7202.2018.01.008 |
| 中文关键词: 银杏蜜环口服溶液 缺血再灌注 脑梗死 神经功能评分 抗氧化能力 |
| English Keywords:Yinxing Mihuan Oral Solution Ischemia/reperfusion Cerebral infarction Neurobehavioral scores Anti-oxidant effect |
| 基金项目:国家自然科学基金项目（81603617）；国家科技重大专项“重大新药创制”（2017ZX09301018） |
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| 中文摘要: |
| 目的：评估银杏蜜环口服溶液对缺血再灌注损伤大鼠脑组织的保护作用，从神经功能评分、脑梗死范围及抗氧化能力探讨银杏蜜环口服溶液保护受损脑组织的药效。方法：首先建立大鼠局灶性脑缺血再灌注模型，将大鼠随机分为假手术组、模型组、金纳多组，银杏叶提取物组，天麻蜜环菌素片组，天麻蜜环菌粉组，银杏蜜环口服溶液618 mg/kg组，银杏蜜环口服溶液309 mg/kg组共8组。除假手术组只分离血管不进行缺血外，其他组大鼠均以线栓阻塞大脑中动脉，1.5 h后再灌注，造成缺血再灌注损伤模型。缺血同时经十二指肠给予各受试药物，假手术和模型组按3 mL/kg体积给予生理盐水。于6 h、24 h进行神经功能评分，末次神经行为评分后取血检测血清中SOD、MDA、GSH-Px水平；取脑后TTC染色，计算脑梗死范围。结果：与模型组比较，银杏蜜环口服溶液组大鼠脑梗死范围显著缩小；大鼠24 h神经功能评分提高；血清SOD及GSH-Px活力增强，MDA水平降低，且优于银杏叶提取物组和天麻蜜环菌组。结论：银杏蜜环口服溶液可减小缺血再灌注大鼠脑梗死范围，减轻神经功能损伤，其抗氧化作用有可能起到神经保护的作用。 |
| English Summary: |
| To evaluate the protective effects of Yinxing Mihuan Oral Solution on brain tissues of ischemia/reperfusion injury model rats, and to explore the pharmaceutical effect in protecting injured brain tissue from neurological deficit score, cerebral infarction range and anti-oxidant effect. Methods: Rats ischemia/reperfusion model was established by nylon thread insertion into middle cerebral artery, and then the model rats were randomized into 8 groups of sham operation group, model group, ginaton group, ginkgo leaf extraction group, gastrodia tuder halimasch tablet group, gastrodia tuder halimasch powder group, Yinxing Mihuan Oral Solution 618 mg/kg group and Yinxing Mihuan Oral Solution 309 mg/kg group. The sham group was only subjected to carotid artery separation without occlusion. Rats in other groups was blocked middle cerebral artery by a thread plug, and reperfusion after 1.5 h, resulting in ischemia-reperfusion injury model. At the same time, drugs were administrated from duodenum with a dose of 3 mL/kg respectively. Sham group was given saline at the same volume instead. Neurological deficit scores were taken one-side blinded 6 h and 24 h after ischemia onset. After the second time of scoring, the rats were sacrificed, and blood sample were taken for serum level examination of SOD, MDA and GSH-PX. Brain was taken for TTC staining to calculate cerebral infarction range. Results: Yinxing Mihuan Oral Solution groups had a significant reduce in cerebral infarction area and MDA level of serum, while a significant increase in 24 h neurological deficit score and serum level of SOD and GSH-PX compared with model group, which was also better than the results of ginkgo leaf extraction group and gastrodia tuder halimasch group. Conclusion: Brain infarction area due to ischemia/reperfusion of rats was reduced by Yinxing Mihuan Oral Solution, with increased neurological deficit scores. The results suggest the brain protective efficacy of Yinxing Mihuan Oral Solution might be related to its anti-oxidative effect. |
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