| 引用本文:彭修娟1，杜志德1，顿宝生1，杨新杰2.高效液相色谱法测定黄七胶囊中人参皂苷Rg1、Rb1及三七皂苷R1的含量[J].世界中医药,2018,(01):. |
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| 高效液相色谱法测定黄七胶囊中人参皂苷Rg1、Rb1及三七皂苷R1的含量 |
| Content Determination of Ginsenoside Rg1, Rb1 and Notoginsenoside R1 in HuangQi Capsules by HPLC |
| 投稿时间：2015-05-14 |
| DOI：10.3969/j.issn.1673-7202.2018.01.051 |
| 中文关键词: 黄七胶囊 高效液相色谱法 人参皂苷Rg1、Rb1 三七皂苷R1 |
| English Keywords:HuangQi Capsules HPLC Ginsenoside Rg1 and Rb1 Notoginsenoside R1 |
| 基金项目:咸阳市2014年重大科技专项（2014k01-21） |
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| 中文摘要: |
| 目的：优选流动相参数，建立高效液相色谱法（HPLC）测定复方制剂黄七胶囊中人参皂苷Rg1、Rb1及三七皂苷R1含量的方法。方法：以十八烷基硅烷键合硅胶为填充剂，以乙腈为流动相A，以水为流动相B，考察最佳梯度洗脱参数。结果：最佳梯度为0～30 min，乙腈19→40，水81→60。在此条件下，人参皂苷Rg1进样量在0.849 6～7.646 4 μg，Rb1在0.853 8～8.853 8 μg，三七皂苷R1在0.202 0～2.020 0 μg范围内皆与峰面积呈良好的线性关系。结论：该流动相梯度洗脱比例提高了峰面积和理论塔板数，并节省了时间。 |
| English Summary: |
| To choose optimal flow phase parameters and establish a content determining of ginsenoside Rg1, Rb and notoginsenoside R1 in HuangQi Capsules by HPLC. Methods:The octadecyl silane chemically bonded silica was used as filler, acetonitrile as mobile phase A and water as mobile phase B. Results:The optimum gradient：0～30 min，acetonitrile：19→40，and water：81→60. On this condition, the sample size of ginsenoside Rg1：0.8496～7.6464 μg, Rb1：0.8538～8.8538 μg, and notoginsenoside R1：0.2020～2.0200 μg, were all in good liner relation with peak area. Conclusion:The mobile phase gradient elution scale improved peak area and number of theoretical plates, and saves time. |
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