世界中医药

作者	单位
郭思远1，马宝伟1，吴晓毅1，李佳1，高伟1，黄璐琦2	1 首都医科大学中医药学院中医络病研究北京市重点实验室，北京，100069； 2 中国中医科学院中药资源中心道地药材国家重点实验室培育基地，北京，100700

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中文摘要:

目的：测定雷公藤属植物叶片中5种萜类成分的含量并比较差异，研究3个物种间的化学亲缘关系。方法：采用HPLC法测定28批雷公藤属植物叶片中雷公藤甲素、雷公藤内酯酮、雷酚新内酯、雷酚内酯和雷公藤红素5个成分的含量，并进行方差分析和聚类分析。结果：雷公藤属植物叶片中，雷公藤甲素、雷酚内酯和雷公藤红素的含量差异有统计学意义（P<0.05）；雷公藤内酯酮和雷酚新内酯含量差异无统计学意义（P>0.05）。运用系统聚类将28批雷公藤属植物聚为4类:第I类为东北雷公藤和昆明山海棠；第II类为雷公藤；第III类为雷公藤；第IV类为雷公藤和昆明山海棠。结论：雷公藤属植物叶片中部分萜类成分含量差异明显。东北雷公藤与雷公藤化学亲缘关系较远；昆明山海棠混在2者之间，与2者无明显区分。

English Summary:

To study the relative relationship among the three species of Tripterygium genus by measuring the contents of 5 terpenoid compositions in plant leaves of Tripterygium genus and comparing the differences.Methods:HPLC was used to measure the 5 composition contents including Triptolide,Triptonide,Neotriptophenolide,Triptophenolide and Tripterine in 28 batches of plant leaves of Triptergium genus.ANOVA and cluster analysis were also performed to verify the results.Results:There were significant differences among the contents of Triptolide,Triptophenolide and Tripterine in the plant leaves of Triptergium genus (P<0.05); There was no significant difference between Triptonide and Neotriptophenolide.Hierarchical clustering was used to divide the 28 batches of plants of Triptergium genus into 4 kinds:I:Tripterygium regelii Sprague et Takeda and Tripterygium hypoglaucum; II:Tripterygium; III:Tripterygium; IV:Tripterygium and Tripterygium hypoglaucum.Conclusion:The differences of some contents of terpenoid compositions in plant leaves of Tripterygium genus were significant.The relative relationship of Tripterygium regelii Sprague et Takeda and Tripterygium was distant; Tripterygium hypoglaucum was between the two,which had no significant difference.

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