To solve the problem of authentication difficulty of saigae tataricae cornu tampon due to loss of traditional identify features such as “Tongtianyan”,and to establish an accurate authentication method for saigae tataricae cornu tampon.Methods:Specific PCR primers for S.tatarica were designed based on cytochrome coxidase I (COI) sequences and the annealing temperature of PCR reaction conditions was optimized,which was performed to authenticate saigae tataricae cornu tampon and its 7 adulterants.Results:Only saigae tataricae cornu tampon could obtain a ~300 bp specific band in electrophoresis while the adulterants did not when the temperature was 64 ℃ and 33 cycle number.Conclusion:Specific PCR could accurately and effectively authenticate the biological origin of sample is S.tatarica or not.