| To investigate the induction effect of abscisic acid (ABA) on the terpenoid secondary metabolites in the suspension cells of Tripterygium wilfordii ,and to explore the hormesis of abscisic acid.Methods:After being subcultured for 10 days,the suspension cells of Tripterygium wilfordii were induced by ABA at different concentrations (10 μM,20 μM,50 μM,100 μM,150 μM,200 μM,500 μM),and the blank control group was set for contrast.They were repeated for 4 times.The HPLC and UPLC was used to detect the samples at 0 h and 240 h after induction,respectively,and the contents of triptolide,triptophenolide,celastrol and wilforlide A were determined in suspension cells.Results:When the concentration of ABA was 10 μM and 20 μM,there was no difference between the induction group and the control group.At 50 μM,the content of triptophenolide significantly increased and the content of celastrol increased to 2.4 times,compared with the control group.And the content of wilforlide A increased to 4.7 times; when the induction concentration exceeded 100 μM,compared with the control group,except wilforlide A,content of triptolide,triptophenolide and celastrol were significantly lower.Conclusion:The effect of ABA on different terpenoids of Tripterygium wilfordii differs from 10 μM to 500 μM.From the overall trend,ABA shows a β-curve,which is in line with the hormesis effect.The 50 μM of ABA significantly enhances the accumulation of celastrol,which laid the foundation for later study on the mechanism of ABA affecting tripterine synthesis. |
