To observe the protective effect of “combination treatment of the gallbladder and spleen” on gastrointestinal mucosa barrier function in patients with malignant obstructive jaundice.Methods:A total of 60 patients with malignant obstructive jaundice who underwent PTCD treatment were randomly divided into control group and study group.Control group was provided with the supporting treatment such asprotection of the liver, prevention of hemorrhage and anti-infectious methods.The study group received the additional combination treatment of the gallbladder and spleen according to the theories of Zhang Xichun.The effect of such treatment on the gastrointestinal mucosa barrier function in the patients was evaluated by the measurements of some factors.The LAC/MAN in the urine, the levels of endotoxin, D-acid and DAO in the blood were measured.Results:Compared with control group, the LAC/MAN in the urine, endotoxixn, Dlactic acid and DAO levels in the blood decreased significantly in the study group after 7 days treatment (P<0.05) ［the control group was respectively (0.176±0.038), (32.47±11.68)pg/mL, (7.27±2.35)Mm/L, (2.39±0.53)Ku/L］; ［the study group was (0.216±0.029), (19.79±6.59)pg/mL, (4.31±1.25)Mm/L, (1.68±0.35)Ku/L］.A statistically significant difference can be seen between the two groups (P<0.05).Conclusion:“The combination treatment of the gallbladder and spleen” has pronounced effects in the protection of gastrointestinal mucosa barrier in the patients with malignant obstructive jaundice.