To investigate the effects of Duzhong Bushen Jiangu Granules in the treatment of patients with nonunion on serum markers and hemorheology. Methods:A total of 74 cases of tibia fracture nonunion were admitted and treated in Rizhao Hospital of TCM from January 2014 to December 2015. The patients were randomly divided into 2 groups by simple randomization with 37 cases in each group. The patients in control group were treated with interlocking intramedullary nail and conventional treatment, and the observation group patients were treated on the basis of the control group, who were given the administration of Duzhong Bushen Jiangu Granules. The indexes of bone morphogenenic protein-2 (BMP-2), insulin-like growth factor-1 (IGF-1), soluble intracellular cell adhesion molecule-1 (sICAM-1), soluble vascular cell adhesion molecule (sVCAM-1), vascular endothelial growth factor (VEGF), specific alkaline phosphatase (ALP) levels and blood rheology indexes, as well as statistics of 2 groups of callus formation time were recorded before and after 1 month's, 2 months' treatment of the two groups. Results:BMP-2, IGF-1, VEGF, and ALP in the observation group after 1 month's and 2 months' treatment were significantly higher than those in the control group at the same time (P<0.05); compared with the control group, sICAM-1, sVCAM-1, whole blood viscosity, whole blood viscosity and erythrocyte aggregation index at the same time decreased significantly (P<0.05); compared with the control group, small dose of callus, medium dose of callus, a large number of callus and clinical healing time decreased significantly (P<0.05). Conclusion:Duzhong Bushen Jiangu Granules combined with interlocking intramedullary nail in treatment of tibial fracture nonunion can accelerate fracture healing, which has clinical popularized values.