| 引用本文:余渊1，程杰2.玉米须总皂苷通过调节炎性反应因子TNF-α和IL-6改善2型糖尿病大鼠脂代谢[J].世界中医药,2018,(02):. |
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| 玉米须总皂苷通过调节炎性反应因子TNF-α和IL-6改善2型糖尿病大鼠脂代谢 |
| Improvement of Lipid Metabolism of Type 2 Diabetes Rats by Zea Mays L.Saponin (ZMLS) Through Regulating Tumor Necrosis Factor-α (TNF-α) and InterLeukin-6 (IL-6) |
| 投稿时间：2016-11-21 |
| DOI：10.3969/j.issn.1673-7202.2018.02.042 |
| 中文关键词: 玉米须总皂苷 肿瘤坏死因子-α 白介素-6 2型糖尿病 大鼠 |
| English Keywords:Zea mays L.saponin (ZMLS) Tumor Necrosis Factor-α InterLeukin-6 Type 2 Diabetes Rat |
| 基金项目:云南省应用基础研究计划项目(2015HS048) |
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| 中文摘要: |
| 目的:探讨分析玉米须总皂苷(Saponin Extracted from Zea Mays L,ZMLS)对高脂饮食加链脲佐菌素(Streptozocin,STZ)诱导的2型糖尿病大鼠脂代谢的影响,以为临床中本病的治疗提供理论基础。方法:60只雄性SD大鼠随机分为正常组,2型糖尿病组以及玉米须总皂苷观察组,每组20只,2型糖尿病模型建立成功后,玉米须总皂苷观察组给予玉米须总皂苷4 mg/(kg·d)灌胃治疗,正常组和2型糖尿病组给生理盐水灌胃比较,治疗4周和8周后处死大鼠各半,HE染色检测肝脏脂肪蓄积特点,试剂盒检测血脂和抗氧化指标,RT-PCR和Western Blot检测肝脏TNF-α和IL-6表达特点。结果:HE染色可见,与正常组比较,模型组有明显脂肪空泡,提示2型糖尿病大鼠有明显的肝脏脂肪代谢异常,玉米须总皂苷治疗后,其脂肪空泡明显减少;与正常组比较,模型组血糖、血脂TG、氧化因子MDA明显升高(P<0.05),胰岛素、血脂HDL-C和抗氧化指标SOD明显下降(P<0.05),玉米须总皂苷治疗4周后,TG和MDA明显下降(P<0.05),HDL-C和SOD明显升高(P<0.05),治疗8周后进一步改善(P<0.05);RT-PCR和Western Blot可见与正常组比较,模型组TNF-α和IL-6表达明显升高(P<0.05),治疗4周后有一定降低,但改善并不明显,治疗8周后均明显降低(P<0.05)。结论:玉米须总皂苷对2型糖尿病大鼠脂代谢具有明显的调节作用,作用机制可能与其可调节TNF-α和IL-6表达有关。 |
| English Summary: |
| To study the effects of Zea mays L.saponin (ZMLS) on type 2 diabetes rats induced by high fat diet and Streptozocin (STZ),which provided a fundamental basis for the treatment of the disease.Methods:A total of 60 male SD rats were randomly divided into normal group,type 2 diabetes group (model groups),and ZMLS group,with 20 cases in each group.Rats in ZMLS group were given ZMLS 4 mg/kg/day by gavage treatment,rats in normal group and model group were given physiological saline by gavage.After treatment for 4 weeks and 8 weeks,half of the rats were sacrificed,HE staining was used to detect the Liver fat accumulation characteristics,and lipid and antioxidant indexes were detected by the kit.The expression of TNF-α and IL-6 in liver tissue were detected by RT-PCR and Western Blot.Results:HE staining showed that there were obvious fat vacuoles in the model group compared with the normal group,which showed an obvious abnormality of liver fat metabolism in type 2 diabetes rats.After treatment,the fat vacuoles decreased significantly.Compared with the normal group,the blood sugar,TG and MDA in the model group were significantly increased (P<0.05); insulin,HDL-C and SOD were decreased (P<0.05).After 4 weeks of ZMLS treatment,the levels of TG and MDA were significantly decreased (P<0.05) and the levels of insulin,HDL-C and SOD were significantly increased (P<0.05),which further improved after 8 weeks of treatment (P<0.05); Compared with the normal group by RT-PCR and Western Blot,the expression of TNF-α and IL-6 in the model group was significantly increased (P<0.05) and then decreased after 4 weeks treatment,but the improvement was not obvious.After 8 weeks of treatment,all the indexes significantly reduced (P<0.05).Conclusion:ZMLS can significantly regulate blood lipid of rats with type 2 diabetes,which maybe related with its regulating function of the expression of TNF-α and IL-6. |
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