| 引用本文:沈水杰，徐玲俊.养肺控瘤方对Lewis晚期肺癌小鼠的抑瘤作用及对白介素-2和肿瘤坏死因子-α表达的影响[J].世界中医药,2018,(02):. |
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| 养肺控瘤方对Lewis晚期肺癌小鼠的抑瘤作用及对白介素-2和肿瘤坏死因子-α表达的影响 |
| Tumor-inhibitory Effect of Yangfei Kongliu Fang on Mice with Advanced Lewis Lung Carcinoma and Its Effects on the Expression of IL-2 and TNF-α |
| 投稿时间：2017-03-27 |
| DOI：10.3969/j.issn.1673-7202.2018.02.043 |
| 中文关键词: 养肺控瘤方；Lewis晚期肺癌 小鼠；抑瘤作用；白介素-2；肿瘤坏死因子-α |
| English Keywords:Yangfei Kongliu Fang, Mouse with advanced Lewis lung carcinoma, Tumor-inhibitory effect, IL-2, TNF-α |
| 基金项目:2015年南通市卫生局青年医学人才科研基金项目（WQ2015066） |
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| 中文摘要: |
| 目的：观察养肺控瘤方对Lewis晚期肺癌小鼠模型的抑瘤作用及对血清白介素-2（IL-2）、肿瘤坏死因子-α（TNF-α）表达的影响。方法：选取30只C57BL/6雄性小鼠，采用胸腔注射Lewis肺癌细胞悬液制作Lewis晚期肺癌小鼠。造模后随机分为6组：空白对照组、顺铂组、等效剂量中药组、高剂量中药组、顺铂+等效剂量中药组、顺铂+高剂量中药组，每组5只。计算各组抑瘤率，并采用酶联免疫吸附试验检测血清中IL-2、TNF-α的表达。结果：顺铂组、等效剂量中药组、高剂量中药组、顺铂+等效剂量中药组、顺铂+高剂量中药组的抑瘤率分别为57.52%，30.88%，51.15%，67.73%，82.75%。与空白对照组比较，各观察组血清IL-2、TNF-α水平均得到显著下降(P<0.05），顺铂+高剂量中药组与顺铂组比较，血清IL-2、TNF-α水平显著下降（P<0.05）。结论：养肺控瘤方能抑制肿瘤生长，并能与顺铂协同降低Lewis晚期肺癌小鼠模型血清IL-2、TNF-α的表达。 |
| English Summary: |
| To observe the tumor-inhibitory effect of Yangfei Kongliu Fang and its effect on the expression of serum IL-2 and TNF-α in mouse models with advanced Lewis lung carcinoma. Methods:By using Lewis lung carcinoma cell suspensions via intrapleural injection, 30 male C57BL/6 mice were prepared into mouse models with advanced Lewis lung carcinoma. After model establishment, the mice were randomly divided into 6 groups: normal saline group (group C/control group), cisplatin group (DDP group), group treated with an equivalent dose of Chinese medicinals (group L), group treated with a high dose of Chinese medicinals (group H), group treated with cisplatin+equivalent dose of Chinese medicinals (group L+DDP), and group treated with cisplatin+high dose of Chinese medicinals (group H+DDP). Each group contained 5 mice and underwent corresponding intervention. Then, the tumor inhibition rate in each group was calculated, and the ELISA method was used to measure the expression of serum IL-2 and TNF-α. Results:The tumor inhibition rates in the DDP group, group L, group H, group L+DDP and group H+DDP were 57.52%, 30.88%, 51.15%, 67.73% and 82.75%, respectively. Compared with the control group, each treatment group achieved significant decreases in the levels of serum IL-2 and TNF-α (P<0.05); and in group H+DDP as compared with DDP group, there were significant decreases in the levels of serum IL-2 and TNF-α (P<0.01). Conclusion:Yangfei Kongliu Fang can inhibit tumor growth and can cooperate with cisplatin to reduce the expression of serum IL-2 and TNF-α in mouse models with advanced Lewis lung carcinoma. |
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