To study the effects of Acaí on neurotransmitter,triaxial hormone and cyclic nucleotide in rats with deficiency heat syndrome and to discuss the drug property of cold and heat.All the SD rats were divided into blank group,deficiency-heat model group,deficiency-heat + Cortex Phellodendri group,deficiency-heat + Acaí high dose and low dose groups.The model rats were prepared by using daily intramuscular injection of dexamethasone sodium phosphate with continuous injection for 21 days.The changes of neurotransmitter (NE,DA,5-ht),hormones (T4,T3,CORT,E2,T) and cyclic nucleotide (cAMP and cGMP) were detected by enzyme-free method and free-release method.The level of NE,DA,T4,T3,E2,cAMP and cGMP in serum were significantly decreased in the Acaí high dose group (P<0.05,P<0.001,P<0.001,P<0.01,P<0.001,P<0.001,P<0.001).The levels of 5-HT,CORT and T in serum were significantly increased in the Acaí high dose group (P<0.001,P<0.05,P<0.05).The Acaí showed the same effect with Cortex Phellodendri and Gegen Qinlian Decoction in adjusting the levels of deficiency-heat rats.This experiment verified a comparative pharmacological method to derive the drug property of cold and heat of Asai.