世界中医药

目的:分析苦苣菜水提物对机体炎性反应相关通路的调节作用,并进一步对作用机制进行研究。方法:雄性健康SD小鼠100只,随机分成10组,每组10只,5组进行耳肿实验,5组进行足肿实验。耳肿实验与足肿实验中,分别设置模型组、地塞米松(2 mg/kg)阳性药物组、苦苣菜水提物0.26 g/10 g剂量组、苦苣菜水提物0.13 g/10 g剂量组、苦苣菜水提物0.065 g/10 g剂量组。检测不同组间血清及炎性组织液中COX-2、p38 MAPK、TNF-α及IL-6水平。结果:不同剂量组间小鼠耳廓肿胀度存在差异,各组肿胀抑制率分别为73.12%、43.62%、28.83%、9.61%。与模型组比较,苦苣菜水提物不同剂量组小鼠耳廓肿胀均受到不同程度的抑制,且随着给药剂量的增大,抑制效果逐渐增强。不同剂量组间小鼠足跖肿胀度存在差异;各组肿胀抑制率分别为54.80%、35.09%、29.73%、17.46%。与模型组比较,苦苣菜水提物不同剂量组小鼠足跖肿胀均受到不同程度的抑制,且随着给药剂量的增大,抑制效果逐渐增强。苦苣菜水提物对小鼠炎性组织液中炎性反应相关因子的影响:阳性对照组、苦苣菜水提物中剂量与高剂量组的COX-2、p38 MAPK、TNF-α、IL-6水平与模型组比较,差异有统计学意义(P<0.05)。苦苣菜水提物对小鼠血清中炎性反应相关因子的影响:阳性对照组、苦苣菜水提物中剂量的COX-2、p38 MAPK水平与模型组比较,差异有统计学意义(P<0.05);阳性对照组、苦苣菜水提物中剂量与高剂量组的TNF-α、IL-6水平与模型组比较,差异有统计学意义(P<0.05)。结论:苦苣菜水提物的抗炎机制涉及到炎性反应发生过程中的多个环节和炎性反应因子,有望研发成为有效的抗炎新药,为防治炎性反应性疾病提供新的药用和食疗资源。

English Summary:

To analyze water extract of Sonchus Oleraceus on the regulation of related pathways of body's inflammatory, and further investigate the mechanism of it. Methods:A total of 100 healthy male SD mice were randomly divided into 10 groups (n＝10). Five groups of mice were treated with ear swelling experiment, and foot swelling experiment was used in mice of another five groups. In ear swelling test and foot swelling experiments, model group and dexamethasone group (2 mg/kg) positive drug group, water extract of Sonchus 0.26 g/10 g dose group, water extract of Sonchus 0.13 g/10 g dose group and water extract of Sonchus 0.065 g/10 g group. The levels of COX-2, p38 MAPK, TNF-α and IL-6 in different serum and inflammatory tissue fluid of different groups were detected. Results:There were significant differences of ear swelling degree in different dose groups. The inhibition mice were 73.12%, 43.62%, 28.83%, respectively. Compared with the model group, mice auricle swelling with water extract of Sonchus oleraceus of different dose was inhibited, and with the increase of the dose, inhibition effect was enhanced. There were significant differences in the swelling degree of paw in different dose groups, and the inhibition rates were 54.80%, 35.09%, 29.73% respectively in each group. Compared with the model group, mice paw swelling with Sonchus oleraceus water extract of different dosage were inhibited, and with the increase of the dose, inhibition effect was enhanced. Effects of water extracted of Sonchus oleraceus related factors on the inflammatory tissue of mice in the solution: positive control group, Sonchus oleraceus water extracted dose and high dose group with COX-2, p38 MAPK, TNF-α, IL-6 levels compared with the model group, and there was significant difference (P<0.05). Effects of water extract of Sonchus oleraceus of inflammation related factors in sera of mice: positive control group, Sonchus oleraceus water extract in dose of COX-2 and p38 MAPK levels compared with the model group, and there was significant difference (P<0.05); The positive control group, water extract of Sonchus oleraceus middle dose group and high dose group of TNF-α and IL-6 levels compared with the model group, there was significant difference (P<0.05). Conclusion:Anti-inflammatory mechanism of water extract of Sonchus oleraceus involves many aspects and inflammatory factors in the process of inflammation, which is expected to be developed into new anti-inflammatory drugs effectively, providing new medicinal and therapeutic resources for the prevention and treatment of inflammatory diseases.

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