| 引用本文:杜鹏1，顿宝生2，杨新杰3，彭修娟2.高效液相色谱-蒸发光散射检测器测定黄七胶囊中黄芪甲苷的含量[J].世界中医药,2018,(02):. |
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| 高效液相色谱-蒸发光散射检测器测定黄七胶囊中黄芪甲苷的含量 |
| Contents of Astragaloside in Huangqi Capsules Measured by HPLC-ELSD |
| 投稿时间：2015-01-12 |
| DOI：10.3969/j.issn.1673-7202.2018.02.053 |
| 中文关键词: 黄七胶囊 高效液相色谱-蒸发光散射检测器法 黄芪甲苷 |
| English Keywords:Huangqi Capsules HPLC-ELSD Astragaloside |
| 基金项目:咸阳市2014年重大科技专项（2014k01-21） |
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| 中文摘要: |
| 目的:评价高效液相色谱-蒸发光散射检测器法（HPLC-ELSD）黄七胶囊中黄芪甲苷的含量测定。方法:以十八烷基硅烷键合硅胶为填充剂；以乙腈-水（32:68）为流动相；蒸发光散射检测器；柱压11.2～12.3 MPa；流速：1.0 mL/min；柱温：室温。理论塔板数以黄芪甲苷峰计算应不低于4 000；峰拖尾因子为1.02。结果:HPLC-ELSD法精密度、重复性、稳定性、回收率良好。黄芪甲苷对照品进样量在1.0528～10.528 μg范围内线性关系良好(r=0.999 9,n＝7)。结论:高效液相色谱-蒸发光散射检测器测定黄七胶囊中黄芪甲苷含量是可行的。 |
| English Summary: |
| To evaluate contents of astragaloside in Huangqi Capsules measured by HPLC-ELSD. Methods:The octadecyl silane bonding silica gel was the filler, with acetonitrile-water (32:68) as mobile phase, and the detector was ELSD with the column pressure of 11.2~12.3 MPa and the flow rate of 1.0 mL/min. Column temperature: indoor temperature. Theoretical plate number was calculated by puerarin peak which should not be less than 4 000; Trailing factor was 1.02. Results:The method of HPLC-ELSD had good accuracy, repeatability, stability and recovery rate. A good linearity relationship of the sample size of asreagaloside comparison products in the range of 1.0528-10.528 μg (r=0.999 9, n=7). Conclusion:Contents of astragaloside in Huangqi Capsules measured by HPLC-ELSD is feasible. |
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