高效液相色谱-蒸发光散射检测器测定黄七胶囊中黄芪甲苷的含量
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咸阳市2014年重大科技专项(2014k01-21)


Contents of Astragaloside in Huangqi Capsules Measured by HPLC-ELSD
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    摘要:

    目的:评价高效液相色谱-蒸发光散射检测器法(HPLC-ELSD)黄七胶囊中黄芪甲苷的含量测定。方法:以十八烷基硅烷键合硅胶为填充剂;以乙腈-水(32:68)为流动相;蒸发光散射检测器;柱压11.2~12.3 MPa;流速:1.0 mL/min;柱温:室温。理论塔板数以黄芪甲苷峰计算应不低于4 000;峰拖尾因子为1.02。结果:HPLC-ELSD法精密度、重复性、稳定性、回收率良好。黄芪甲苷对照品进样量在1.0528~10.528 μg范围内线性关系良好(r=0.999 9,n=7)。结论:高效液相色谱-蒸发光散射检测器测定黄七胶囊中黄芪甲苷含量是可行的。

    Abstract:

    To evaluate contents of astragaloside in Huangqi Capsules measured by HPLC-ELSD. Methods:The octadecyl silane bonding silica gel was the filler, with acetonitrile-water (32:68) as mobile phase, and the detector was ELSD with the column pressure of 11.2~12.3 MPa and the flow rate of 1.0 mL/min. Column temperature: indoor temperature. Theoretical plate number was calculated by puerarin peak which should not be less than 4 000; Trailing factor was 1.02. Results:The method of HPLC-ELSD had good accuracy, repeatability, stability and recovery rate. A good linearity relationship of the sample size of asreagaloside comparison products in the range of 1.0528-10.528 μg (r=0.999 9, n=7). Conclusion:Contents of astragaloside in Huangqi Capsules measured by HPLC-ELSD is feasible.

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杜鹏,顿宝生,杨新杰,彭修娟.高效液相色谱-蒸发光散射检测器测定黄七胶囊中黄芪甲苷的含量[J].世界中医药,2018,(02).

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  • 收稿日期:2015-01-12
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  • 在线发布日期: 2018-03-23
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