世界中医药

目的：对蒙药手参药材和奶手参（奶制饮片）进行系统性生药学鉴定及分析，为其质量标准的建立及鉴定提供科学依据。方法：参照《中华人民共和国药典》（2010年版附录）相关方法，对手参药材和奶手参的水分，灰分及浸出物进行测定；采用显微，薄层色谱法进行定性鉴别。以天麻素为对照品，用70%甲醇提取，以乙酸乙酯-甲酸-水（9:1:0.2）为展开剂，喷以10%磷钼酸乙醇溶液，在105 ℃加热至斑点显色清晰。采用紫外-可见分光光度法和高效液相色谱法对手参与奶手参中的多糖及天麻素进行含量分析。色谱柱为ZOBB AX eclipse XDB-C18(4.6 mm×250 mm,5 cm)柱；以甲醇-0.04%的磷酸溶液（8:92）为流动相，流速为1.0 mL/min；检测波长222 nm;柱温30 ℃。结果：薄层色谱法试验中在与对照品色谱相应的位置上，显相同颜色的斑点。高效液相色谱条件下天麻素被洗脱且达到基线分离，天麻素在浓度0.009～0.09 mg/mL范围内线性关系良好，回归方程为Y=586866X+425821（R2=0.9996），平均回收率为100.1%。精密度试验、重复性试验、稳定性试验均符合要求。三批样品浸出物结果为26.13%～42.58%,水分为3.47%～5.31%，总灰分为5.43%～6.33%。结论：质控方法可靠，灵敏度高，专属性，准确，稳定性好。该结果对手参药材鉴定、资源利用及质量标准提升，提供科学依据。

English Summary:

To carry out a systematic pharmacognostical study on Shoushen and Naishoushen, and to provide the basis for establishing its quality standard and appraisal. Methods:Based on the methods recorded in appendix of Pharmacopoeia of the People's Republic of China (2010 edition, volume1), the water content, total ash and ethanol-soluble extractives of the crude drugs were detected. The microscopy TLC was used for qualitative identification. With gastrodin as control agent, 70% methyl alcohol was used to extract. With ethyl acetate formic acid-water (9:1:0.2) as developing solvent, 10% phosphomolybdic acid ethanol solution was sprayed. And at 105 ℃ heated until spot color was clear. By ultraviolet-visible spectrophotometry and HPLC method were used for the determination of contents of the polysaccharides and gastrodine of Shoushen and Naishoushen. Chromatographic column for ZOBB AX eclipse XDB-C18 (4.6 mm×250 mm, 5 cm); methanol-water (0.04% phosphoric acid) (8:92) was used as the mobile phase at a flow rate of 1.0 mL/min. The column temperature was 30 ℃ and the detection wavelength was at 222 nm. Results:In thin layer chromatography (TLC), the same color spots were detected in the corresponding positions of the chromatography. HPLC was used to elute the gastrodine and achieve the baseline separation. The concentration of gastrodine in the concentration range 0.009～0.09 mg/mL was good. And the regression equation was Y=586866X+425821 (R2=0.9996) and the average recovery was 100.1%. Precision test, repeatability test, stability test were in line with the requirements. Three batches of sample extract was 26.13%～42.58%, water 3.47%～5.31% and total ash content was 5.43%～6.33%. Conclusion:The indictment method is reliable, sensitive, specific, accurate and stable. The results provide scientific basis for identification, resource utilization and improvement of quality standards.

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