To establish the quality standard of traditional Mongolian Sumu Liuwei Tangsan. Methods:Lignum Sappan, Radix Aucklandiae, Semen Arecae and Rhizoma Alpiniae Officinarum were identified by TLC qualitatively. The content of brazilin and protosappanin B in Lignum Sappan was determined by HPLC. The separation was performed on Topsil C18 (4.6 mm×250 mm, 5 μm) with mobile phase consisted of acetonitrile-water (11:89) at the flow rate of 1.0 mL/min. The detection wavelength was set at 285 nm. The column temperature was 30 ℃. The sample size was 10 μL. Results:The TLC spots of Lignum Sappan, Radix Aucklandiae, Semen Arecae and Rhizoma Alpiniae Officinarum were clear and specific. The linear range was 112.875-564.375 ng for brazilin (r=0.9997) and 71.975-359.875 ng for protosappanin B (r=0.9999) respectively. The RSDs of precision, stability and repeatability tests were no more than 2%. The average recoveries were respectively 99.24 % (RSD=0.12 %, n=6) and 98.06 % (RSD=0.93 %, n=6). Conclusion:The qualitative identification is specific and reproducible, and quantitative method is simple, accurate and reliable, which can be used for the quality control of traditional medicine Sumu Liuwei Tangsan.