世界中医药

目的：探讨比较黄芩水煎液及黄芩苷对肝纤维化大鼠的防治作用及量效关系。方法：采用皮下注射四氯化碳（CCL4）8周诱导大鼠肝纤维化模型，并同时灌胃给予高、中、低剂量的黄芩水煎液及黄芩苷，给药结束后，检测大鼠肝脏病理组织形态；试剂盒检测肝脏HYP，血清肝功能指标(ALT、AST、ALP、GGT)及肝组织匀浆及血清SOD、MDA水平；放免法检测血清肝纤维化指标(HA、PC-Ⅲ、C-Ⅳ及LN)。结果：与模型组比较，中、低剂量黄芩水煎液及黄芩苷均能有效改善大鼠肝功能，降低血清HA、LN、PCⅢ水平及肝脏HYP含量，提高血清及肝组织SOD，降低MDA水平，并尤以低剂量组作用佳，但黄芩水煎高剂量组具有加重肝损伤趋势。结论：中、低剂量黄芩水煎液及黄芩苷均能有效改善大鼠肝纤维化，其机制可能与其抗氧化能力有关。

English Summary:

To study the therapeutic effect and dose-effect relationship of Raix Scutellariae decoction and Baicalin on hepatic fibrosis (HF) rats. Methods:HF rat model was established by subcutaneous injection of CCL4 for 8 weeks. Meanwhile, different doses of Raix Scutellariae decoction and Baicalin were administered. Histomorphology of liver tissue was observed. Liver HYP from hepatic tissue, the serum levels of ALT, AST, ALP, GGT, the liver and serum levels of SOD and MDA were detected by Kit. The liver fibrosis indexes including HA, PC-Ⅲ, C-Ⅳ and LN were measured by radioimmunoassay. Results:As compared with those in model group, the liver content of HYP, the serum levels of HA, LN, PCⅢ, the serum and liver levels of MDA, SOD were significantly changed in middle and low dose of Raix Scutellariae decoction and Baicalin groups, which also could significantly improve hepatic function. Additionally, the middle and low dosages were beneficial. However, the high dosage of Raix Scutellariae decoction could obviously aggravate liver injury. Conclusion:Middle and low dose of Raix Scutellariae decoction and Baicalin can effectively improve HF in rats and the action may be completed by antagonizing lipid peroxidation.

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