世界中医药
文章摘要
引用本文:麦荣国,郭晓銮,招丽娟,林家寿,刘少文,张建伟.麻黄汤不同配伍对苦杏仁和甘草有效成分的影响[J].世界中医药,2018,(03):.  
麻黄汤不同配伍对苦杏仁和甘草有效成分的影响
Study on the Effects of Different Compatibility of Mahuang Decoction on the Active Ingredients of Semen Armeniacae Amarum and Radix Glycyrrhizae Based on Fingerprint
投稿时间:2017-07-17  
DOI:10.3969/j.issn.1673-7202.2018.03.048
中文关键词:  指纹图谱  麻黄汤  不同配伍  苦杏仁  甘草  苦杏仁苷  甘草酸单铵盐  高效液相色谱法
English Keywords:Fingerprint  Mahuang decoction  Different compatibility  Semen Armeniacae Amarum  Radix Glycyrrhizae  Amygdalin  Mono ammonium salt of glycyrrhizic acid  HPLC
基金项目:佛山市卫生局(2012155)
作者单位
麦荣国,郭晓銮,招丽娟,林家寿,刘少文,张建伟 广东省佛山市中医院制剂中心,佛山528216 
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中文摘要:
      目的:评价麻黄汤不同配伍对苦杏仁和甘草有效成分的影响。方法:制备苦杏仁苷对照品溶液和甘草酸单铵盐对照品溶液和不同配伍方式的麻黄汤水煎液供试品溶液,以正交设计8中不同配伍的麻黄汤组方,苦杏仁有效成分研究中以A-H进行标识;甘草有效成分研究中以A1-H1对8中不同配伍方式进行标实。最终结果根据国家药典委员会提出的中药指纹图谱相似度评价系统2004A进行相似度分析,采用MATLAB软件进行数据处理和统计。结果:苦杏仁和甘草色谱条件能够分别将苦杏仁苷和甘草酸清晰的分离出来,无其他杂质峰,基线稳定,具有可比性。麻黄汤不同配伍方式中对苦杏仁有效成分苦杏仁苷每剂含量未见明显差异,进行方差分析后结果显示,麻黄汤不同配伍中麻黄、桂枝、甘草对苦杏仁苷的影响差异无统计学意义(P>005)。麻黄汤不同配伍方式中以H1单纯甘草中甘草酸含量为最高,而麻黄汤全方A1中的甘草酸含量为最低;不同配伍中麻黄、桂枝和苦杏仁均降低麻黄汤中甘草酸的含量(P<005)。结论:麻黄汤不同配伍中麻黄、桂枝、甘草对苦杏仁中有效成分苦杏仁苷未见明显影响;而不同配伍中麻黄、桂枝和苦杏仁均降低麻黄汤中甘草有效成分甘草酸的含量。
English Summary:
      To evaluate the effects of different compatibility of Mahuang decoction on the effective components of Semen Armeniacae Amarum and Radix Glycyrrhizae based on the fingerprint of Chinese herbal medicine. Methods:Amygdalin reference solution and glycyrrhizinic acid standard solution and the different combination of Mahuang decoction test solution were prepared. 8 types of different combinations of Mahuang decoction were orthogonally designed, and A-H mark was used to effective ingredients of Semen Armeniacae Amarum; A1-H1 was marked to active ingredients of Radix Glycyrrhizae in different combinations Mahuang Decoction. Finally, the similarity analysis was carried out, according to the similarity evaluation system 2004A of traditional Chinese medicine fingerprints proposed by the national pharmacopoeia committee, and the MATLAB software was used for data processing and statistics. Results:1) The chromatographic conditions of Semen Armeniacae Amarum and Radix Glycyrrhizae could separate the amygdalin and glycyrrhizic acid respectively. There was no other impurity peak, and the baseline was stable and comparable. 2) There was no significant difference in amygdalin of Semen Armeniacae Amarum in each different Mahuang decoction. The variance analysis showed that there was no significant difference (P>005) in Herba Ephedrae, Ramulus Cinnamomi, Radix Glycyrrhizae in Mahuang decoction with different compatibilities. 3) H1 with pure glycyrrhizin content was the highest in different combination of Mahuang Decoction, while the contents of A1 with glycyrrhizic acid in Mahuang decoction were the lowest. Herba Ephedrae, Ramulus Cinnamomi, Radix Glycyrrhizae in different combination of Mahuang Decoction decreased the content of glycyrrhizic acid in Mahuang decoction (P<005). Conclusion:Herba Ephedrae, Ramulus Cinnamomi and Radix Glycyrrhizae in different compatibility of Mahuang decoction had little significant effects on almond amygdalin; and they all decreased the contents of effective components of licorice of glycyrrhizic acid.
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