世界中医药
文章摘要
引用本文:陈露露1,林汝秀1,李瑞明2.小柴胡汤不同煎煮方式对黄岑有效化学成分的影响[J].世界中医药,2018,(03):.  
小柴胡汤不同煎煮方式对黄岑有效化学成分的影响
Effects of Different Decoction Methods of Xiaochaihu Decoction on Active Chemical Constituents of Scutellaria Baicalensis Georgi
投稿时间:2017-09-08  
DOI:10.3969/j.issn.1673-7202.2018.03.050
中文关键词:  小柴胡汤  煎煮  方式  黄岑  有效  化学成分
English Keywords:Xiaochaihu decoction  Decoction method  Scutellaria baicalensis Georgi  Effective chemical composition
基金项目:广东省重大科技专项项目(2011A080300004)
作者单位
陈露露1,林汝秀1,李瑞明2 1 南方医科大学南方医院药剂科广州510515 2 中山大学附属第七医院药学部深圳518107 
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中文摘要:
      目的:探析不同煎煮方式对小柴胡汤中黄岑有效化学成分的影响。方法:选取高效液相色谱法测定小柴胡汤用去滓再煎法与不去滓煎法2种煎煮后的黄岑有效化学成分(黄岑苷、黄岑素、汉黄岑素)的含量。对照品溶液包括黄岑苷、黄岑素、汉黄岑素,检测开始前先进行对照品溶液的线性关系考察及精密度试验、稳定性试验、回收率试验。结果:黄岑苷、黄岑素、汉黄岑素对照品溶液的标准曲线方程分别为Y=4 345 73098X-8 88811(r=0999 8)、Y=2 478 26868X91105(r=0999 5)、Y=771 988 974X2 44903(r=0999 9)。黄岑苷、黄岑素、汉黄岑素对照品溶液的精密度试验、稳定性试验与回收率试验的RSD均低于2%,平均回收率均高于95%。供试品溶液A中所测得的黄岑苷、黄岑素、汉黄岑素含量分别为(0364 2±0027 1)g/L、(10192 3±0387 5)g/L、(18082 2±0746 5)μg/mL,高于供试品溶液B的(0282 4±0047 8)g/L、(8215 0±0354 1)g/L、(12698 2±0642 9)μg/mL(P<005)。结论:去滓再煎法较之传统不去滓煎法更利于小柴胡汤中黄岑有效化学成分黄岑苷、黄岑素、汉黄岑素的溶出。
English Summary:
      To study the effects of different decoction methods on the effective chemical composition of Scutellaria baicalensis Georgi in Xiaochaihu Decoction. Methods:The contents of effective chemical composition (Baicalin, Baicalein, Wogonin) in Xiaochaihu decoction boiled by deslagging and non-deslagging and then decocting were determined by high performance liquid chromatography. The comparison solution included the Baicalin, Baicalein and Wogonin. The linear relationship of the comparison solution and the precision test, the stability test and the recovery rate test were carried out before the start of the test. Results:The standard curve equation of Baicalin, Baicalein and Wogonin comparison solution were Y=4 345 73098X888 811 (r=0999 8), Y=247 826 868X91105 (r=0999 5) and Y=771 988 974X244 903 (r=0999 9). The RSD of Baicalin, Baicalein, Wogonin reference solution in the precision test, stability test and the recovery test were all lower than 2% and the average recoveries were all higher than 95%. The contents of Baicalin, Baicalein and Wogonin in test solution A were (0364 2±0027 1)mg/mL, (10192 3±0387 5)mg/mL and (18082 2±0746 5)μg/mL, which were higher than (0282 4±0047 8)mg/mL, (8215 0±0354 1)mg/mL and (12698 2±0642 9)μg/mL in test solution B (P<005). Conclusion:The deslagging and then decocting were more beneficial to the dissolution of Baicalin, Baicalein, Wogonin in Xiaochaihu decoction compared to the non-deslagging and then decocting.
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