世界中医药

目的:观察细辛水煎液及挥发油抗抑郁作用。方法：将初筛过的小鼠随机分为模型组、阳性组、细辛挥发油组，细辛水煎液高、中、低剂量组(2、1、0.5 g/mL)6组，每组小鼠10只，灌胃给药连续7 d，1次/d，第7天灌胃结束后1 h，进行行为学实验：小鼠悬尾实验(TST)、强迫游泳实验(FST)和开场实验（OFT），观察细辛水煎液及挥发油对小鼠抑郁行为的干预作用。结束后处死小鼠，用Elisa法测定小鼠脑内五羟色胺(5-HT)、谷氨酸(Glu)，γ-氨基丁酸(GABA)的含量。结果：1）细辛水煎液高剂量及细辛挥发油可使小鼠在悬尾实验TST中不动时间显著性缩短(高剂量P<0.01，挥发油P<0.01)；细辛水煎液中剂量及细辛挥发油可使小鼠在强迫游泳实验FST的不动时间均显著性缩短(P<0.05)。2）细辛挥发油剂量组和水煎液高、中、低剂量组结果与模型组比较均能显著增加小鼠脑内GABA的含量(P<0.01)；细辛水煎液高、中剂量组与模型组比较能显著增高小鼠脑内Glu的含量(P<0.05)；细辛水煎液中、低剂量组与模型组相能显著增加小鼠脑内5-HT的含量(中剂量P<0.01，低剂量P<0.05)。结论：首次证实细辛水煎液及挥发油具有抗抑郁作用。细辛挥发油和水煎液在小鼠悬尾模型和强迫游泳模型上均表现出抗抑郁作用，细辛水煎剂和挥发油与盐酸氟西汀作用机制有相似之处。作用机制可能与提高脑内类神经递质5-HT、Glu、GABA含量有关，尤其是与提高单胺类神经递质5-HT浓度关系更加密切。

English Summary:

To observe the antidepressant effect of Radix et Rhizoma Asari decoction and volatile oil.Methods:The screened mice were randomly divided into 6 groups with 10 rats in each group，respectively model group，positive control group，Radix et Rhizoma Asari volatile oil group，Radix et Rhizoma Asari decoction of high，medium and low dose group (2，1，0.5 g/mL).Each group were intragastric administration for 7 d，1 time a day.1 h after the last administration，the tail suspension test (TST)，forced swimming test (FST) and open field test (OFT) were performed to observe the intervention effect of decotion and volatile oil of Radix et Rhizoma Asari on depression behavior of mice.Then the mice were killed，and Enzyme linked immunosorbent assay (ELISA) was used to determine the contents of GABA，glutamate (Glu) and 5-HT in the brain of mice.Results:The high dose and the volatile oil of Radix et Rhizoma Asari could significantly reduce the immobility time of mice in TST (P<0.01，P<0.01).Radix et Rhizoma Asari decoction medium dose and volatile oil could significantly shorted the immobility time of mice in FST (P<0.05).Compared with model group，the volatile oil and decoction of high，medium and low dose group could significantly increase the content of GABA in the brain (P<0.01)，and Radix et Rhizoma Asari decoction high and medium dose group could significantly increase Glu content in the brain (P<0.05).Radix et Rhizoma Asari decoction medium and low dose group could significantly increase the content of 5-HT in the mouse brain (medium dose P<0.01，low dose P<0.05).Conclusion:It is the first time to confirm that Radix et Rhizoma Asari Decoction and volatile oil have antidepressant effect.Radix et Rhizoma Asari volatile oil and decoction both shows antidepressant effect on the model of tail suspension and forced swimming in mice，whose mechanism was consistent with that of fluoxetine hydrochloride.The mechanism of action may be related to increasing the content of GABA，Glu and 5-HT，especially is closely related with 5-HT concentration.

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