世界中医药

目的:观察桑寄生总黄酮（TFHT）对佐剂性关节炎大鼠的药理作用。方法:雄性SD大鼠，测量1次/7 d足趾肿胀度及进行1次关节评分。第22天给药后将大鼠处死，取左后足踝关节（除正常给药组大鼠外）做病理切片观察；酶联免疫吸附测定法测定血清中TNF-α、IL-10、IL-6的含量。结果:TFHT高、中剂量组能够明显改善佐剂性关节炎组大鼠足趾肿胀程度及大鼠全身症状；降低免疫器官系数；降低血清中IL-6、TNF-α的含量，升高IL-10的含量；病理切片显示踝关节软骨破坏明显减轻，渗出物及血管翳生成减少或无；提示桑寄生总黄酮具有显著的祛风湿和逆转骨关节破坏的作用。结论:桑寄生总黄酮具有明显的祛风湿作用，其作用机制与降低血清中IL-6、TNF-α的含量，升高IL-10的含量有关。

English Summary:

To study the pharmacological effect of total flavonoids of Herba Taxilli (TFHT) on adjuvant arthritis in rats.Methods:All male SD rats were injected with Freund's complete adjuvant on the left foot to establish adjuvant joint model.From the beginning of the 9th day of administration，normal group and model group were given 0.5% sodium carboxymethylcellulose (CMC-Na) solution by gavage，while the positive control group was given tripterygium glycosides suspension 1 mg/mL.TFHT high，middle and low dose group were given 360 mg/kg，180 mg/kg，90 mg/kg CMC-Na solution for 22 days.The left rear ankle joint diameter of rats were measured and graded every 7 days from the 15th day of modeling.After 22th day，the rats were sacrificed，and the left hind ankle joints (except the normal administration group) were observed for pathological sections.TNF-α，IL-10 and IL-6 in serum were determined by Elisa.Results:TFHT high and middle dose group significantly improved the degree of swelling of the toes and the systemic symptoms of rats in adjuvant arthritis group; decreased the immune organ coefficient; decreased the levels of IL-6 and TNF-α，increased IL-10 content.Pathological sections showed ankle cartilage destruction was significantly reduced，exudate and pannus formation decreased or even disappered.Pharmacodynamics experiments showed that TFHT had significant rheumatism and reversal of bone and joint damage.Conclusion:TFHT has obvious effects of anti-rheumatism，whose pharmacological mechanisms are related with the reduce of IL-6 and TNF-α，as well as the rise of IL-10 in serum.

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